Document Detail


Suppression subtractive hybridization identifies distinctive expression markers for coronary and internal mammary arteries.
MedLine Citation:
PMID:  12615697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We sought to identify differentially expressed genes in the athero-prone coronary artery and athero-resistant internal mammary arteries.
METHODS AND RESULTS: Using suppressive subtraction hybridization, we generated reciprocal cDNA collections of representative mRNAs specific to porcine coronary arteries versus porcine mammary arteries. We screened 1000 suppressive subtraction hybridization cDNA clones by dot blot array and sequenced 600 of those showing the most marked expression differences. Northern blot, in situ hybridization, and immunostaining confirmed the differential gene expression patterns identified by the dot blot arrays. Genes associated with mammary arteries included claudin-10 and h-cadherin, which are genes associated with tight junctions and intermediate junctions. In contrast, genes associated with proatherosclerotic processes, such as lipid retention and metabolism, inflammation, and cell growth, were preferentially expressed in coronary arteries.
CONCLUSIONS: Normal coronary arteries have gene expression program that is significantly different than internal mammary arteries. These differences may partly explain the resistance of coronary arteries and internal mammary arteries to atherosclerosis.
Authors:
Minghui Qin; Zhaohui Zeng; Jie Zheng; Prediman K Shah; Stephen M Schwartz; Lawrence D Adams; Behrooz G Sharifi
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-01-30
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  23     ISSN:  1524-4636     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-17     Completed Date:  2003-04-11     Revised Date:  2013-04-15    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  425-33     Citation Subset:  IM    
Affiliation:
Atherosclerosis Research Center, Division of Cardiology, and Burns and Allen Research Institute, Cedars-Sinai Medical Center, Davis Bldg #1016, 8700 Beverly Blvd, Los Angeles, Calif 90048, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteriosclerosis / genetics
Blotting, Northern
Cadherins / analysis,  genetics
Caveolin 1
Caveolins / analysis,  genetics
Claudins
Coronary Vessels / metabolism*
DNA Probes
DNA, Complementary / analysis*
Gene Expression*
Immunoblotting
Mammary Arteries / metabolism*
Membrane Proteins / analysis,  genetics
Reference Values
Ribonuclease, Pancreatic / analysis,  genetics
Swine
Tenascin / analysis,  genetics
Grant Support
ID/Acronym/Agency:
HL50566/HL/NHLBI NIH HHS; R01 HL090653-04/HL/NHLBI NIH HHS; R01 HL104068-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins; 0/Caveolin 1; 0/Caveolins; 0/Claudins; 0/DNA Probes; 0/DNA, Complementary; 0/H-cadherin; 0/Membrane Proteins; 0/Tenascin; 0/claudin 10; EC 3.1.27.-/angiogenin; EC 3.1.27.5/Ribonuclease, Pancreatic
Comments/Corrections

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