| Suppression of reactive oxygen species and enhanced stress tolerance in Rubia cordifolia cells expressing the rolC oncogene. | |
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MedLine Citation:
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PMID: 18986252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that expression of the Agrobacterium rhizogenes rolC gene in transformed plant cells causes defense-like reactions, such as increased phytoalexin production and expression of pathogenesis-related proteins. In the present study, we examined whether this phenomenon is associated with increased production of reactive oxygen species (ROS). Single-cell assays based on confocal microscopy and fluorogenic dyes (2,7-dichlorofluorescein diacetate and dihydrorhodamine 123) showed reduced steady-state levels of ROS in rolC-expressing Rubia cordifolia cells as compared with normal cells. Paraquat, a ROS inducer, caused significant ROS elevation in normal cells but had little effect on rolC-transformed cells. Likewise, ROS elevation triggered by a light stress was suppressed in transformed cells. Our results indicate that the rolC gene acts as a ROS suppressor in unstressed cells and its expression prevents stress-induced ROS elevations. We detected a two- to threefold increase in tolerance of rolC-transformed cells to salt, heat, and cold treatments. Simultaneously, rolC-transformed cells maintained permanently active defensive status, as found by measuring isochorismate synthase gene expression and anthraquinone production. Thus, the oncogene provoked multiple effects in which ROS production and phytoalexin production were clearly dissociated. |
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Authors:
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Victor P Bulgakov; Dmitry L Aminin; Yuri N Shkryl; Tatiana Y Gorpenchenko; Galina N Veremeichik; Pavel S Dmitrenok; Yuri N Zhuravlev |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular plant-microbe interactions : MPMI Volume: 21 ISSN: 0894-0282 ISO Abbreviation: Mol. Plant Microbe Interact. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-06 Completed Date: 2008-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107902 Medline TA: Mol Plant Microbe Interact Country: United States |
Other Details:
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Languages: eng Pagination: 1561-70 Citation Subset: IM |
Affiliation:
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Institute of Biology and Soil Science, Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russia. bulgakov@ibss.dvo.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Anthraquinones / metabolism Bacterial Proteins / genetics, metabolism* Cells, Cultured Fluorometry Gene Expression Regulation, Plant Genes, Bacterial Intramolecular Transferases / metabolism Microscopy, Confocal Paraquat / pharmacology Plants, Genetically Modified / genetics, metabolism RNA, Plant / genetics Reactive Oxygen Species / metabolism* Reverse Transcriptase Polymerase Chain Reaction Rhizobium radiobacter / genetics Rubia / drug effects, genetics, metabolism* Salicylic Acid / metabolism Stress, Physiological* Terpenes / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anthraquinones; 0/Bacterial Proteins; 0/RNA, Plant; 0/Reactive Oxygen Species; 0/Terpenes; 37297-20-4/phytoalexins; 4685-14-7/Paraquat; 69-72-7/Salicylic Acid; EC 5.4.-/Intramolecular Transferases; EC 5.4.4.2/isochorismate synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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