| Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. | |
| | |
MedLine Citation:
|
PMID: 19168728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oxidant injury occurs when an organ is severed from its native blood supply and then reperfused and continues during subsequent periods of immune attack. Experiments here test the hypothesis that an antioxidant given only in the peri-reperfusion period protects against not only oxidative but also nitrosative stress, leading to reduced vasculopathy long after cardiac allotransplantation. Experiments were performed using a murine heterotopic cardiac transplantation model. An antioxidant, in the form of intraperitoneal high-dose riboflavin, was given to recipients during the initial 3 days after transplantation. Antioxidant-treated mice showed significantly longer graft survival than control mice. At 4 h after transplantation, antioxidant treatment significantly reduced graft lipid peroxidation and oxidized DNA and preserved antioxidant enzyme activity. At day 6 posttransplantation, the redox-sensitive transcription factor nuclear factor-kappaB and inducible nitric oxide synthase were significantly reduced following antioxidant treatment, with concomitant reduction of nitrotyrosine. Despite the limited duration of antioxidant treatment, both acute and chronic rejection were significantly suppressed. In vitro experiments confirmed suppression of nitrosative and oxidative stress and cardiomyocyte damage in antioxidant-treated cardiac allografts. Collectively, antioxidant administration during the initial 3 days after transplantation significantly reduces nitrosative and oxidative stress in cardiac allografts, modulates immune responses, and protects against vasculopathy. |
| | |
Authors:
|
Tomomi Hasegawa; Koichiro Iwanaga; Donald E Hultquist; Hui Liao; Scott H Visovatti; David J Pinsky |
Related Documents
:
|
8855448 - The anti-oxidant properties of 5-aminosalicylic acid. 20374848 - Effect of natural pigments on the oxidative stability of sausages stored under refriger... 9626578 - Analysis of plasma antioxidant capacity by competition kinetics. 10401618 - The importance of lipid solubility in antioxidants and free radical generating systems ... 16238338 - Zinc(ii) triflate-controlled 1,3-dipolar cycloadditions of c-(2-thiazolyl)nitrones: app... 8855448 - The anti-oxidant properties of 5-aminosalicylic acid. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-01-23 |
Journal Detail:
|
Title: American journal of physiology. Heart and circulatory physiology Volume: 296 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-03-31 Completed Date: 2009-06-10 Revised Date: 2010-09-23 |
Medline Journal Info:
|
Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: H1007-16 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antioxidants / pharmacology Disease Models, Animal Dose-Response Relationship, Drug Graft Survival / drug effects Heart Transplantation / adverse effects*, physiology Male Mice Mice, Inbred C57BL Mice, Inbred CBA NF-kappa B / metabolism Nitric Oxide Synthase Type II / metabolism Nitrosation / drug effects Oxidative Stress / drug effects Reactive Nitrogen Species / antagonists & inhibitors*, metabolism Reactive Oxygen Species / antagonists & inhibitors*, metabolism Reperfusion Injury / etiology, metabolism, prevention & control Riboflavin / pharmacology Transplantation, Homologous Vascular Diseases / etiology*, metabolism, prevention & control* |
| Grant Support | |
ID/Acronym/Agency:
|
P01HL089407/HL/NHLBI NIH HHS; R01HL055397/HL/NHLBI NIH HHS; R01HL085149/HL/NHLBI NIH HHS; T32-HL-007853/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/NF-kappa B; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 83-88-5/Riboflavin; EC 1.14.13.39/Nitric Oxide Synthase Type II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: NADPH oxidase contributes to coronary endothelial dysfunction in the failing heart.
Next Document: Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced art...