Document Detail


Suppression of motion artifacts in fluorescence spectroscopy of perfused hearts.
MedLine Citation:
PMID:  1415626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescence spectroscopy of beating hearts has been used previously to measure intracellular regulators of function. Unfortunately, heart motion could introduce a spurious motion artifact (MA), influencing the measured fluorescence intensity ("signal"). To suppress MA, a ratio (or difference) has been calculated previously between the signal and an intensity reference detected at a different wavelength ("reference"). However, no studies have attempted to evaluate or optimize the efficiency of MA suppression. MA suppression was evaluated using reflected excitation light or fluorescence as reference. In addition, the MA contribution to the intensity ratio from a fluorescent dye, indo-1, was quantified. A reflected light reference resulted in poor suppression of MA. The use of a fluorescence reference resulted in suppression that was inversely related to the detection wavelength difference (delta) between the reference and signal. Therefore optimal MA suppression was obtained using a fluorescence reference at a wavelength close to the signal. For delta = 60 nm, MA was suppressed from approximately 10 to less than 2%. Finally, suppressed MA (delta = 60 nm) accounted for less than 10% of the indo-1 ratio fluctuations.
Authors:
R Brandes; V M Figueredo; S A Camacho; B M Massie; M W Weiner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  263     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-10-29     Completed Date:  1992-10-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H972-80     Citation Subset:  IM    
Affiliation:
Department of Medicine (Cardiology), University of California, San Francisco 94143.
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MeSH Terms
Descriptor/Qualifier:
Animals
Artifacts*
Calcium / metabolism
Diphenylhexatriene
Fluorescent Dyes
Fluorometry / methods*
Heart / physiology*
Indoles
Intracellular Membranes / metabolism
Male
Motion*
Perfusion
Rats
Rats, Sprague-Dawley
Reference Values
Grant Support
ID/Acronym/Agency:
1-F32-HL-08244/HL/NHLBI NIH HHS; 3-PO1-HL-25847/HL/NHLBI NIH HHS; R01-DK-33923/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indoles; 1720-32-7/Diphenylhexatriene; 7440-70-2/Calcium; 96314-96-4/indo-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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