Document Detail

Suppression of metastatic potential of high-metastatic Lewis lung carcinoma cells by vanadate, an inhibitor of tyrosine phosphatase, through inhibiting cell-substrate adhesion.
MedLine Citation:
PMID:  9030244     Owner:  NLM     Status:  MEDLINE    
Treatment of high-metastatic Lewis lung carcinoma A11 cells with sodium orthovanadate, a phosphotyrosine phosphatase inhibitor, resulted in a dose- and time-dependent suppression of cell spreading on various extracellular matrix components such as Matrigel, fibronectin, laminin and type IV collagen, while the treatment did not significantly inhibit attachment of the cells to these substrates. Orthovanadate slightly and reversibly inhibited the in vitro cell growth of A11 cells, but the suppression of cell spreading was not directly due to the inhibition of cell growth. Orthovanadate-treated A11 cells showed reduced invasive ability in a reconstituted basement membrane invasion assay and experimental metastatic ability. Protein tyrosine phosphorylation level in A11 cells was elevated after treatment with orthovanadate. The increase in tyrosine phosphorylation level was partially diminished by the tyrosine kinase inhibitor ST638, concomitantly with restoration of the suppressed cell spreading as well as invasive and metastatic abilities. These results suggest that protein tyrosine phosphorylation influences invasive and metastatic potential of tumor cells possibly through regulating cell-substrate adhesion.
K Takenaga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Invasion & metastasis     Volume:  16     ISSN:  0251-1789     ISO Abbreviation:  Invasion Metastasis     Publication Date:  1996  
Date Detail:
Created Date:  1997-03-07     Completed Date:  1997-03-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8202435     Medline TA:  Invasion Metastasis     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
Division of Chemotherapy, Chiba Cancer Center Research Institute, Japan.
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MeSH Terms
3T3 Cells
Antineoplastic Agents / pharmacology,  therapeutic use*
Carcinoma, Lewis Lung / drug therapy*,  metabolism,  pathology
Cell Adhesion / drug effects*
Cell Size / drug effects
Cinnamates / pharmacology,  therapeutic use
Drug Combinations
Enzyme Inhibitors / pharmacology*
Extracellular Matrix Proteins / biosynthesis,  genetics
Gene Expression Regulation, Neoplastic / drug effects
Lung Neoplasms / pathology,  prevention & control
Mice, Inbred C57BL
Neoplasm Invasiveness
Neoplasm Metastasis / prevention & control*
Neoplasm Proteins / biosynthesis,  genetics
Phosphoproteins / metabolism
Protein Processing, Post-Translational
Protein Tyrosine Phosphatases / antagonists & inhibitors*
Sulfides / pharmacology,  therapeutic use
Tumor Cells, Cultured
Vanadates / pharmacology,  therapeutic use*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Cinnamates; 0/Drug Combinations; 0/Enzyme Inhibitors; 0/Extracellular Matrix Proteins; 0/Laminin; 0/Neoplasm Proteins; 0/Phosphoproteins; 0/Proteoglycans; 0/Sulfides; 0/Vanadates; 107761-24-0/ST 638; 119978-18-6/matrigel; 9007-34-5/Collagen; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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