| Suppression of mammary epithelial cell differentiation by the helix-loop-helix protein Id-1. | |
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MedLine Citation:
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PMID: 7760836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell proliferation and differentiation are precisely coordinated during the development and maturation of the mammary gland, and this balance invariably is disrupted during carcinogenesis. Little is known about the cell-specific transcription factors that regulate these processes in the mammary gland. The mouse mammary epithelial cell line SCp2 grows well under standard culture conditions but arrests growth, forms alveolus-like structures, and expresses beta-casein, a differentiation marker, 4 to 5 days after exposure to basement membrane and lactogenic hormones (differentiation signals). We show that this differentiation entails a marked decline in the expression of Id-1, a helix-loop-helix (HLH) protein that inactivates basic HLH transcription factors in other cell types. SCp2 cells stably transfected with an Id-1 expression vector grew more rapidly than control cells under standard conditions, but in response to differentiation signals, they arrested growth and formed three-dimensional structures similar to those of control cells. Id-1-expressing cells did not, however, express beta-casein. Moreover, 8 to 10 days after receiving differentiation signals, they lost three-dimensional organization, invaded the basement membrane, and then resumed growth. SCp2 cells expressing an Id-1 antisense vector grew more slowly than controls; in response to differentiation signals, they remained stably growth arrested and fully differentiated, as did control cells. We suggest that Id-1 renders cells refractory to differentiation signals and receptive to growth signals by inactivating one or more basic HLH proteins that coordinate growth and differentiation in the mammary epithelium. |
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Authors:
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P Y Desprez; E Hara; M J Bissell; J Campisi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 15 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-06-28 Completed Date: 1995-06-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3398-404 Citation Subset: IM |
Affiliation:
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Department of Cancer Biology, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / drug effects* Cell Line DNA-Binding Proteins / metabolism, pharmacology* Epithelial Cells Epithelium / drug effects Helix-Loop-Helix Motifs* Inhibitor of Differentiation Protein 1 Mammary Glands, Animal / drug effects* Mice Repressor Proteins* Signal Transduction Transcription Factors* |
| Grant Support | |
ID/Acronym/Agency:
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AG10004/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Idb1 protein, mouse; 0/Inhibitor of Differentiation Protein 1; 0/Repressor Proteins; 0/Transcription Factors |
| Comments/Corrections | |
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