Document Detail

Suppression of FAT/CD36 mRNA by human growth hormone in pancreatic β-cells.
MedLine Citation:
PMID:  21679697     Owner:  NLM     Status:  Publisher    
Fatty acid-induced damage in pancreatic β-cells is assumed to play an important role in the development of type 2 diabetes. Lactogens (prolactin, placental lactogen and growth hormone) improve β-cell survival via STAT5 activation but the molecular targets are incompletely characterized. The aim of this study was to examine the effect of human growth hormone (hGH) on mRNAs of fatty acid transport and binding proteins expressed in pancreatic β-cells, and to examine this in relation to β-cell survival after exposure to fatty acids. hGH decreased mRNA levels of FAT/CD36, whereas mRNAs of GPR40, FASN, FABP2, FATP1 and FATP4 were unchanged. RNAi against FAT/CD36 decreased fatty acid-induced apoptosis. Over-expression of constitutively active STAT5 was able to mimic hGH's suppression of FAT/CD36 expression, whereas dominant negative STAT5 was unable to block the effect of hGH indicating that STAT5 did not bind directly to the FAT/CD36 promoter. The hGH-mediated suppression of FAT/CD36 mRNA was associated with a decrease in palmitate uptake and fatty acid-induced basal hyper-secretion of insulin resulting in improved glucose-stimulated insulin secretion. This study suggests that hGH can protect β-cells against fatty acid-induced damages.
Louise T Dalgaard; Peter Thams; Louise W Gaarn; Janne Jensen; Ying C Lee; Jens H Nielsen
Related Documents :
4065187 - Antisecretory effect and oral pharmacokinetics of omeprazole in patients with chronic r...
8415927 - Gastroduodenal mucosal protection.
1645887 - Histamine and gastric acid secretion. a review.
7233077 - Inhibition of pentagastrin-stimulated gastric acid secretion by graded intraduodenal ad...
20101327 - Topical treatment of melasma.
10752667 - The preservatives ethyl-, propyl- and butylparaben are oestrogenic in an in vivo fish a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-7
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Science, Roskilde University, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  H(+)/myo-inositol transporter genes, hmit-1.1 and hmit-1.2, have roles in the osmoprotective respons...
Next Document:  Anticancer activity of thymoquinone in breast cancer cells: Possible involvement of PPAR-? pathway.