Document Detail


Suppression of lymphocyte activation by a protein released from isolated perfused rat liver.
MedLine Citation:
PMID:  6978837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolated rat liver perfusates contain a substance which inhibits 3H-thymidine uptake by phytohemagglutinin-stimulated human peripheral blood lymphocytes in a dose-dependent, noncytotoxic fashion. Suppression is not due to interference of lymphocyte-phytohemagglutinin interaction or dilution of the thymidine pool. Complete inhibition of thymidine uptake is achieved with less than 1.0 microgram of material per ml (which is a potentially achievable concentration in vivo). The release of this material is directly and quantitatively associated with hepatocellular injury as measured by release of glutamic pyruvate transaminase. The material is a highly basic protein with a molecular weight of approximately 65,000 to 80,000 daltons. It is a product of the hepatocyte rather than of nonparenchymal liver cells. Liver-derived materials, such as the presently described molecule, may play a role in in situ regulation of lymphocyte function during immunologically mediated liver disease.
Authors:
C Pizzo; D Lee; F V Chisari
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  2     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:    1982 May-Jun
Date Detail:
Created Date:  1982-07-19     Completed Date:  1982-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-303     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine Transaminase / physiology
Animals
DNA / biosynthesis
Liver / immunology,  physiology*
Lymphocyte Activation*
Male
Mice
Mice, Inbred Strains
Perfusion
Phytohemagglutinins / metabolism
Proteins / physiology*
Rats
Rats, Inbred Lew
Thymidine / metabolism
Grant Support
ID/Acronym/Agency:
AI 13393/AI/NIAID NIH HHS; AI 17353/AI/NIAID NIH HHS; RCDA AI 00174/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Phytohemagglutinins; 0/Proteins; 50-89-5/Thymidine; 9007-49-2/DNA; EC 2.6.1.2/Alanine Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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