Document Detail


Suppression of lipopolysaccharide-induced upregulation of toll-like receptor 4 by emodin in mouse proximal tubular epithelial cells.
MedLine Citation:
PMID:  22735924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the effects of emodin, the major component of Rheum palmatum, on lipopolysaccharide (LPS)-induced toll-like receptor 4 (TLR4) expression in cultured mouse tubular epithelial cells (TECs). The TECs were obtained from mice and incubated with LPS and/or indicated concentrations of emodin for 24 h. Cytokeratin, α-SMA and vimentin were detected using immunohistochemistry. The TLR4 protein level was detected by flow cytometry. TNFα and IL-6 protein levels were measured using an enzyme-linked immunosorbent assay (ELISA). mRNA expression of TLR4, TNFα and IL-6 was detected using a reverse-transcription polymerase chain reaction (RT-PCR). Results showed that a concentration of 102 ng/ml LPS significantly upregulated TLR4 mRNA and protein levels. TNFα and IL-6 mRNA and protein levels were also increased. Emodin (at doses of 40, 20 and 10 µM) was able to inhibit LPS-induced TLR4 protein synthesis in cultured TECs. However, TNFα and IL-6 protein expression was decreased in cells treated with emodin at concentrations of 40 and 20 µM. These results demonstrate that an elevated expression of inflammatory cytokines and TLR4 in cells stimulated with LPS, were simultaneously inhibited by emodin. Emodin is therefore able to inhibit the LPS-induced expression of TLR4 in order to downregulate TNFα and IL-6 synthesis in TECs, which may contribute to the protective effects of emodin in renal disease.
Authors:
Xiao-Ling Zhu; Yong-Jun Wang; Ya-Ζhen Yang; Ru-Chun Yang; Bin Zhu; Ying-Ηua Zhang; Yi Lin; Ying Lu; Xiao-Feng Li; Kevin T O'Byrne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-21
Journal Detail:
Title:  Molecular medicine reports     Volume:  6     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-06     Completed Date:  2012-11-06     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  493-500     Citation Subset:  IM    
Affiliation:
Department of Nephrology, Hangzhou Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310007, PR China. zhuxiaolinghz@126.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Emodin / pharmacology*
Epithelial Cells / drug effects,  metabolism*
Immunohistochemistry
Interleukin-6 / genetics,  metabolism
Kidney Tubules, Proximal / cytology
Lipopolysaccharides / toxicity
Male
Mice
Protein Kinase Inhibitors / pharmacology*
Rheum / chemistry
Toll-Like Receptor 4 / genetics,  metabolism*
Tumor Necrosis Factor-alpha / genetics,  metabolism
Up-Regulation / drug effects
Chemical
Reg. No./Substance:
0/Interleukin-6; 0/Lipopolysaccharides; 0/Protein Kinase Inhibitors; 0/Toll-Like Receptor 4; 0/Tumor Necrosis Factor-alpha; 518-82-1/Emodin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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