Document Detail


Suppression of leaf feeding and oviposition of phytophagous ladybird beetles (Coleoptera: Coccinellidae) by chitinase gene-transformed phylloplane bacteria and their specific bacteriophages entrapped in alginate gel beads.
MedLine Citation:
PMID:  12852588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chitinase gene-transformed strain KPM-007E/chi of Enterobacter cloacae was vitally entrapped in sodium alginate gel beads with its specific virulent bacteriophage EcP-01 to provide a new method for microbially digesting chitinous peritrophic membranes of phytophagous ladybird beetles Epilachna vigintioctopunctata. First, chitinase SH1 from a gram-positive bacterium Kurthia zopfii was overproduced by Escherichia coli cells and purified by affinity column chromatography. The purified enzyme effectively digested peritrophic membranes dissected from the ladybird beetles to expose epithelial tissues beneath the peritrophic membrane, and the beetles that had ingested chitinase after submergence in chitinase solution had considerably reduced their feeding on tomato leaves. KPM-007E/chi, entrapped in the alginate beads, released the chitinase. More chitinase was released when KPM-007E/chi was present with their specific virulent bacteriophage EcP-01 in the beads because of lysis of bacterial cells infected with the bacteriophages. This chitinase release from the microbial beads (containing KPM-007E/chi and EcP-01) was sufficient to digest the peritrophic membrane as well as to suppress feeding of bead-sprayed tomato leaves by the ladybird beetles. A daily supply of tomato leaves treated with the microbial beads considerably suppressed leaf feeding and oviposition by the ladybird beetles, suggesting a possible application of chitinase-secreting bacteria for suppressing herbivorous insect pests.
Authors:
Yasunari Otsu; Hirofumi Mori; Kenji Komuta; Hiroyuki Shimizu; Souta Nogawa; Yoshinori Matsuda; Teruo Nonomura; Yasuyuki Sakuratani; Yukio Tosa; Shigeyuki Mayama; Hideyoshi Toyoda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of economic entomology     Volume:  96     ISSN:  0022-0493     ISO Abbreviation:  J. Econ. Entomol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-10     Completed Date:  2004-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985127R     Medline TA:  J Econ Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  555-63     Citation Subset:  IM    
Affiliation:
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara, 631-8505 Japan.
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MeSH Terms
Descriptor/Qualifier:
Alginates
Animals
Bacteriophages / physiology
Beetles / physiology*
Chitinase / genetics,  metabolism*
Enterobacter cloacae / enzymology*,  genetics,  virology*
Feeding Behavior*
Gels
Insect Control / methods*
Lycopersicon esculentum / parasitology*
Microspheres
Oviposition*
Plant Leaves
Transformation, Bacterial
Chemical
Reg. No./Substance:
0/Alginates; 0/Gels; EC 3.2.1.14/Chitinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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