Document Detail


Suppression of hindlimb inputs to S-I forelimb-stump representation of rats with neonatal forelimb removal: GABA receptor blockade and single-cell responses.
MedLine Citation:
PMID:  10848556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neonatal forelimb removal in rats results in the development of inappropriate hindlimb inputs in the forelimb-stump representation of primary somatosensory cortex (S-I) that are revealed when GABA(A) and GABA(B) receptor activity are blocked. Experiments carried out to date have not made clear what information is being suppressed at the level of individual neurons. In this study, three potential ways in which GABA-mediated inhibition could suppress hindlimb expression in the S-I stump representation were evaluated: silencing S-I neurons with dual stump and hindlimb receptive fields, silencing neurons with receptive fields restricted to the hindlimb alone, and/or selective silencing of hindlimb inputs to neurons that normally express a stump receptive field only. These possibilities were tested using single-unit recording techniques to evaluate the receptive fields of S-I forelimb-stump neurons before, during, and after blockade of GABA receptors with bicuculline methiodide (for GABA(A)) and saclofen (for GABA(B)). Recordings were also made from normal rats for comparison. Of 92 neurons recorded from the S-I stump representation of neonatally amputated rats, only 2.2% had receptive fields that included the hindlimb prior to GABA receptor blockade. During GABA receptor blockade, 54.3% of these cells became responsive to the hindlimb, and in all but two cases, these same neurons also expressed a stump receptive field. Most of these cells (82.0%) expressed only stump receptive fields prior to GABA receptor blockade. In 71 neurons recorded from normal rats, only 5 became responsive to the hindlimb during GABA receptor blockade. GABA receptor blockade of cortical neurons, in both normal and neonatally amputated rats, resulted in significant enlargements of receptive fields as well as the emergence of receptive fields for neurons that were normally unresponsive. GABA receptor blockade also resulted in increases in both the spontaneous activity and response magnitudes of these neurons. These data support the conclusion that GABA mechanisms generally act to specifically suppress hindlimb inputs to S-I forelimb-stump neurons that normally express a receptive field on the forelimb stump only.
Authors:
A S Stojic; R D Lane; H P Killackey; R W Rhoades
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  83     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-12     Completed Date:  2000-07-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3377-87     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Neurobiology, Medical College of Ohio, Toledo, Ohio 43699, USA.
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MeSH Terms
Descriptor/Qualifier:
Amputation Stumps / physiopathology*
Animals
Animals, Newborn / physiology*
Baclofen / analogs & derivatives,  pharmacology
Bicuculline / pharmacology
Cytoplasmic Granules / physiology
Forelimb / innervation*
GABA Antagonists / pharmacology*
Hindlimb / innervation*
Motor Cortex / cytology,  drug effects,  physiology
Neurons / drug effects*
Rats
Receptors, GABA-A / antagonists & inhibitors
Receptors, GABA-B / antagonists & inhibitors
Grant Support
ID/Acronym/Agency:
DE-07734/DE/NIDCR NIH HHS; NS-28888/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/GABA Antagonists; 0/Receptors, GABA-A; 0/Receptors, GABA-B; 1134-47-0/Baclofen; 125464-42-8/saclofen; 485-49-4/Bicuculline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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