Document Detail

Suppression of hepatitis B virus enhancer 1 and 2 by hepatitis C virus core protein.
MedLine Citation:
PMID:  12445429     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Epidemiological studies have shown that coinfection or superinfection with hepatitis B virus (HBV) and C virus (HCV) frequently leads to the suppression of hepatitis B virus replication. The mechanism of this phenomenon is still unclear. Shih et al. [J Virol 1993;67:5823] reported a direct suppression of HBV replication by the core protein of HCV. The target structure of HCV core protein in this system remained unclear. METHODS: As HCV core protein has been shown to influence expression from transcriptional elements, we studied whether HCV core protein altered the activity of the two HBV enhancers 1 and 2. Luciferase vectors for HBV enhancers 1 or 2 were cotransfected with expression constructs for HCV core protein in murine and human hepatocyte lines. RESULTS: Full-length HCV core protein suppressed the HBV enhancer 1 up to 11-fold, the enhancer 2 3-4-fold. Suppression of HBV enhancer 1 by HCV core from genotype 1b was stronger than by HCV core of genotypes 3a or 1a. Carboxyterminally truncated core proteins had lower or no suppression activity. CONCLUSIONS: These data suggest that HCV core protein may directly repress transcription of the HBV RNAs. This trans-repression may contribute to suppression of HBV replication in patients coinfected with both viruses.
Christian G Schüttler; Nicola Fiedler; Katja Schmidt; Reinald Repp; Wolfram H Gerlich; Stephan Schaefer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of hepatology     Volume:  37     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-26     Completed Date:  2003-06-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  855-62     Citation Subset:  IM    
Institut für Medizinische Virologie Justus-Liebig-Universität, Frankfurter Strasse 107, D-35392 Giessen, Germany.
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MeSH Terms
Cell Line
Enhancer Elements, Genetic / drug effects*
Hepatitis B virus / genetics*,  metabolism*
Hepatocytes / metabolism
Peptide Fragments / pharmacology
Tumor Cells, Cultured
Viral Core Proteins / chemistry,  pharmacology*
Reg. No./Substance:
0/Peptide Fragments; 0/Viral Core Proteins

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