Document Detail

Suppression of gastric mucosal inflammatory responses to Helicobacter pylori lipopolysaccharide by sulglycotide.
MedLine Citation:
PMID:  10188628     Owner:  NLM     Status:  MEDLINE    
The effect of the antiulcer agent sulglycotide on gastric epithelial cell apoptosis and the expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-4 (IL-4) during Helicobacter pylori lipopolysaccharide-induced acute gastritis was investigated. Rats, pretreated twice daily for 3 consecutive days with sulglycotide at 200 mg/kg or vehicle, were subjected to surface epithelial application of H. pylori lipopolysaccharide (50 microg/animal), and, after 4 additional days on the drug or vehicle regimen, their mucosal tissue was used for histologic assessment, quantitation of TNF-alpha and IL-4, and the assay of epithelial cell apoptosis. In the absence of sulglycotide, H. pylori lipopolysaccharide caused acute mucosal responses manifested by the inflammatory infiltration of the lamina propria with lymphocytes and plasma cells, edema, hyperemia, and epithelial hemorrhage. These responses were accompanied by an 11-fold increase in epithelial cell apoptosis and a 9-fold enhancement of the mucosal expression of proinflammatory cytokine TNF-alpha. However, the mucosal expression of regulatory cytokine IL-4 decreased by 15%. Treatment with sulglycotide produced significant (56.6%) reduction in the extent of acute mucosal inflammatory changes caused by H. pylori lipopolysaccharide. Moreover, the effect of sulglycotide was manifested in an 88.3% reduction in the epithelial cell apoptosis and a 69.1% decrease in the mucosal expression of TNF-alpha, whereas the expression of IL-4 showed only marginal (6%) enhancement. The results suggest that the cytoprotective agent sulglycotide suppresses the inflammatory and apoptotic events elicited in gastric mucosa by H. pylori lipopolysaccharide through stimulation of TNF-alpha expression.
B L Slomiany; J Piotrowski; A Slomiany
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  General pharmacology     Volume:  32     ISSN:  0306-3623     ISO Abbreviation:  Gen. Pharmacol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-26     Completed Date:  1999-05-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7602417     Medline TA:  Gen Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  251-7     Citation Subset:  IM    
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400, USA.
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MeSH Terms
Anti-Ulcer Agents / pharmacology,  therapeutic use*
Endothelium / drug effects,  pathology
Gastric Mucosa / drug effects*,  metabolism,  pathology
Helicobacter pylori / chemistry
Lipopolysaccharides / pharmacology
Rats, Sprague-Dawley
Sialoglycoproteins / pharmacology,  therapeutic use*
Stomach Diseases / chemically induced,  prevention & control*
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/Lipopolysaccharides; 0/Sialoglycoproteins; 54182-59-1/sulglicotide

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