Document Detail

Suppression of first cleavage in the Mexican axolotl (Ambystoma mexicanum) by heat shock or hydrostatic pressure.
MedLine Citation:
PMID:  7338728     Owner:  NLM     Status:  MEDLINE    
Androgenetic diploid axolotls were produced by ultraviolet inactivation of the egg pronucleus shortly after fertilization, followed by suppression of the first cleavage division by hydrostatic pressure or heat shock. After treatment at 14,000 psi for 8 minutes, diploidy was restored in 74% of the embryos, but only 0.8% survived to hatching. A 36-37 degrees C heat shock of 10-minutes duration, applied 5.5 hours after the eggs were collected, yielded a slightly lower percentage of diploids. However, the proportion surviving to hatching was significantly greater (up to 4.6%). A second generation of androgenetic diploids was produced from one of the oldest of the first generation males with a similar degree of success. The lack of significant improvement suggests that the low survival is due to the heat shock per se and not to the uncovering of recessive lethal genes carried by the parent.
L L Gillespie; J B Armstrong
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  218     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-06-14     Completed Date:  1982-06-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  441-5     Citation Subset:  IM    
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MeSH Terms
Ambystoma / embryology*
Cell Division / drug effects,  radiation effects
Cleavage Stage, Ovum* / drug effects,  radiation effects
Cytochalasin B / pharmacology
Hot Temperature*
Hydrostatic Pressure*
Ultraviolet Rays
Reg. No./Substance:
14930-96-2/Cytochalasin B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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