Document Detail


Suppression of error prone pathway is responsible for antimutagenic activity of honey.
MedLine Citation:
PMID:  22269905     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Honey, both unifloral (Syzygiumcumini) and bifloral, demonstrated strong antimutagenicity against physical (UV, γ) and chemical (ethylmethane sulfonate) mutagens as ascertained by rpoB/Rif(R) and Ames tests. The effect of honey was evaluated in radiation (UV or γ) exposed Escherichia coli cells for SOS response, a well known error prone repair pathway known to significantly contribute to mutagenicity by quantifying LexA repressor level, measuring cell filamentation frequency, and prophage induction by SIVET (Selectable - In-Vivo Expression Technology) assay. LexA was almost completely degraded, phenotypically long filamentous cells (∼30μm) were formed, and SIVET induction frequency was increased in radiation exposed E. coli cultures, however, these changes were significantly inhibited in presence of honey confirming its strong antimutagenic nature. Further, rpoB/Rif(R) mutation frequency upon UV exposure in E. coli recA(-) cells was found to be negligible, whereas, E. coliumuC(-) and umuD(-) knockouts showed comparatively higher mutation frequency. Honey did not show any effect on mutagenesis in these knockouts, indicating the SOS dependence of the observed mutagenesis. Honey was also found to suppress EMS induced mutagenesis but through SOS independent mechanism. Phenolics present in honey were found to be one of the important factors contributing to the antimutagenicity of honey.
Authors:
Sudhanshu Saxena; Satyendra Gautam; Girish Maru; Dhananjay Kawle; Arun Sharma
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-14
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
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