Document Detail


Suppression of corneal graft rejection by subconjunctival injection of FK-506 in a rat model of penetrating keratoplasty.
MedLine Citation:
PMID:  7543958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The usefulness of the subconjunctival injection of FK-506 for suppression of allograft rejection was investigated in a rat model of orthotopic penetrating keratoplasty. Fischer rats were used as donors and Dark Agouti rats, as recipients. FK-506 was administered subconjunctivally in a dosage of 0.3 mg/kg/day for 15 consecutive days after penetrating keratoplasty was performed. Allograft rejection occurred within 8 to 10 days after keratoplasty in all untreated rats (n = 6). None of the FK-506-treated rats (n = 6) exhibited graft rejection during the 3-week observation period. Histologic examination showed marked infiltration of mononuclear cells in the stroma of corneal grafts from untreated rats 3 weeks after grafting. Inflammatory cells were only occasionally observed in grafts from FK-506-treated rats. Donor-specific cytotoxic T lymphocyte activity was completely suppressed in FK-506-treated rats 3 weeks after grafting. Our results indicated that subconjunctival injection of FK-506 effectively prevented corneal allograft rejection in a rat model of penetrating keratoplasty.
Authors:
A Minamoto; H Sakata; K Okada; M Fujihara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of ophthalmology     Volume:  39     ISSN:  0021-5155     ISO Abbreviation:  Jpn. J. Ophthalmol.     Publication Date:  1995  
Date Detail:
Created Date:  1995-09-18     Completed Date:  1995-09-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0044652     Medline TA:  Jpn J Ophthalmol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  12-9     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Conjunctiva / drug effects*
Cornea / immunology,  pathology
Cytotoxicity, Immunologic / drug effects
Disease Models, Animal
Drug Administration Schedule
Graft Rejection / immunology,  pathology,  prevention & control*
Injections
Keratoplasty, Penetrating*
Male
Rats
Rats, Inbred F344
Rats, Inbred Strains
T-Lymphocytes, Cytotoxic / immunology
Tacrolimus / administration & dosage,  pharmacology*
Transplantation, Homologous
Chemical
Reg. No./Substance:
109581-93-3/Tacrolimus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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