Document Detail

Suppression of chimpanzee NORS in hamster/chimpanzee hybrid: report on cell line R48-26.
MedLine Citation:
PMID:  10696238     Owner:  NLM     Status:  MEDLINE    
We present the first documented NOR suppression in a hybridoma other than man-mouse for the hamster-chimpanzee hybrid cell line R48-26. Alu PCR and chromosome painting showed that in this cell line chimpanzee chromosomes 13-15-23 are maintained. NORs on chimpanzee chromosomes 15-23, whose presence was directly verified by FISH with H 28s rDNA, resulted inactive while telomeric rDNA on hamster chromosomes resulted active even if hamster chromosomes presented extensive rearrangements. We observed an all or nothing model in accordance with a model of regulation by selective transcriptional factors. The rearrangements of hamster chromosomes have not involved the location of NORs because they maintain a telomeric position.
P Cavagna; R Marzella; M Rocchi; B Chiarelli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  24     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  2000-03-10     Completed Date:  2000-03-10     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  303-6     Citation Subset:  IM    
Istituto di Antropologia Università di Firenze.
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MeSH Terms
CHO Cells
Cell Line
Chromosome Painting
Cricetulus / genetics*
DNA, Ribosomal / genetics
Gene Expression Regulation, Neoplastic / genetics*
Hybrid Cells / metabolism
In Situ Hybridization, Fluorescence
Nucleolus Organizer Region / genetics*
Pan troglodytes / genetics*
RNA, Ribosomal, 28S / genetics
Telomere / genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Ribosomal; 0/RNA, Ribosomal, 28S

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