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Suppression by antisense mRNA demonstrates a requirement for the glial fibrillary acidic protein in the formation of stable astrocytic processes in response to neurons.
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MedLine Citation:
PMID:  1999469     Owner:  NLM     Status:  MEDLINE    
The glial fibrillary acidic protein (GFAP) is a glial-specific intermediate filament protein, which is expressed in astrocytes in the central nervous system, as well as in astrocytoma cell lines. To investigate the function of GFAP, we have studied the human astrocytoma cell line, U251, which constitutively expresses GFAP and vimentin in the same 10-nm filaments. These cells respond to neurons in vitro in the same way as primary astrocytes: they withdraw from the cell cycle, support neuronal cell survival and neurite outgrowth, and they extend complex, GFAP-positive processes. To determine the role of GFAP in these responses, we have specifically suppressed its expression by stably transfecting the U251 cells with an antisense GFAP construct. Two stable antisense cell lines from separate transfections were isolated and were shown to be GFAP negative by Northern and Western blot analyses, and by immunofluorescence studies. The antisense cell lines were inhibited in their ability to extend significant glial processes in response to neurons. In culture with primary neurons, the average increase in process length of the U251 cells was nearly 400%, as compared to only 14% for the antisense transfectants. The other neuron induced responses of astrocytes, i.e., proliferative arrest and neuronal support, were not affected in these cell lines. These data support the conclusion that the glial-specific intermediate filament protein, GFAP, is required for the formation of stable astrocytic processes in response to neurons.
D E Weinstein; M L Shelanski; R K Liem
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  112     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-04-10     Completed Date:  1991-04-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1205-13     Citation Subset:  IM    
Department of Pathology, Columbia University College of Physicians and Surgeons, New York 10032.
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MeSH Terms
Animals, Newborn
Axons / physiology
Cell Communication*
Cell Line
Cell Survival
Cell Transformation, Viral
Cells, Cultured
Glial Fibrillary Acidic Protein / genetics,  physiology*
Neurons / cytology,  physiology*
RNA, Antisense / genetics*
RNA, Messenger / genetics*
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/RNA, Antisense; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 3 Year: 1991
Volume: 112 Issue: 6
First Page: 1205 Last Page: 1213
ID: 2288905
Publisher Id: 91154343
PubMed Id: 1999469

Suppression by antisense mRNA demonstrates a requirement for the glial fibrillary acidic protein in the formation of stable astrocytic processes in response to neurons

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