Document Detail

Suppression of artifacts induced by homonuclear decoupling in amino-acid-type edited methyl 1H-13C correlation experiments.
MedLine Citation:
PMID:  15388081     Owner:  NLM     Status:  MEDLINE    
A detailed theoretical and experimental analysis of the artifacts induced by homonuclear band-selective decoupling during CT frequency labeling is presented. The effects are discussed in the context of an amino-acid-type editing filter implemented in (1)H-(13)C CT-HSQC experiments of methyl groups in proteins. It is shown that both Bloch-Siegert shifts and modulation sidebands are efficiently suppressed by using additional off-resonance decoupling as proposed by Zhang and Gorenstein [J. Magn. Reson. 132 (1998) 81], and appropriate adjustment of a set of pulse sequence parameters. The theoretical predictions are confirmed by experiments performed on (13)C-labeled protein samples, yielding artifact-free amino-acid-type edited methyl spectra.
Hélène Van Melckebeke; Jean-Pierre Simorre; Bernhard Brutscher
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of magnetic resonance (San Diego, Calif. : 1997)     Volume:  170     ISSN:  1090-7807     ISO Abbreviation:  J. Magn. Reson.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-24     Completed Date:  2004-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9707935     Medline TA:  J Magn Reson     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A.-UJF 41, rue Jules Horowitz, 38027 Grenoble Cedex, France.
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MeSH Terms
Amino Acids / chemistry*
Calmodulin / chemistry*
Carbon Isotopes
Hydrogen Bonding
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*
Reg. No./Substance:
0/Amino Acids; 0/Calmodulin; 0/Carbon Isotopes; 0/Nitrogen Isotopes

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