Document Detail

Suppression of allograft rejection in the sponge Suberites domuncula by FK506 and expression of genes encoding FK506-binding proteins in allografts.
MedLine Citation:
PMID:  11507104     Owner:  NLM     Status:  MEDLINE    
Porifera (sponges) are, evolutionarily, the oldest metazoan phylum. Recent molecular data suggest that these animals possess molecules similar to and homologous with those of the innate and adaptive immune systems of higher Metazoa. Applying the biological system of parabiosis and the technique of differential display of mRNA, two cDNAs encoding putative FK506-binding proteins were isolated. FK506 is successfully used in clinics as a drug to prevent allograft rejection and is toxic to Suberites domuncula cells in vitro at doses above 100ng ml(-1). Autograft fusion of transplants from S. domuncula was not affected by FK506. Allograft non-fusion was not affected by FK506 at toxic doses; however, at the non-toxic dose of 20ng ml(-1), the allografts fused with each other. It is shown that at the attachment zone in untreated and (particularly drastic) in FK506-treated allografts, expression of the genes encoding the FK506-binding proteins is upregulated. These data indicate that the drug FK506 suppresses allograft rejection in S. domuncula, most probably via interaction with expression of the gene coding for the FK506-binding proteins.
W E Müller; R Steffen; B Lorenz; R Batel; M Kruse; A Krasko; I M Müller; H C Schröder
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  204     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-16     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2197-207     Citation Subset:  IM    
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany. WMUELLER@mail.UNI-Mainz.DE
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ278328;  AJ278329
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MeSH Terms
Amino Acid Sequence
Cloning, Molecular
DNA, Complementary / genetics
Gene Expression / drug effects*
Gene Library
Graft Rejection* / prevention & control
Molecular Sequence Data
Polymerase Chain Reaction
Porifera / drug effects,  genetics*,  immunology
Sequence Homology
Tacrolimus / pharmacology*
Tacrolimus Binding Proteins / chemistry,  genetics*
Transplantation, Homologous / immunology
Reg. No./Substance:
0/DNA, Complementary; 109581-93-3/Tacrolimus; EC 5.2.1.-/Tacrolimus Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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