Document Detail


Suppression of acetate mutants in Coprinus : I. Identification of two isoaccepting tRNA suppressors of a missense mutation.
MedLine Citation:
PMID:  24173445     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The acu-1 gene of Coprinus is the structural gene for acetyl-CoA synthetase. Cell free extracts of acu-1.4 mutants lack detectable enzyme activity. Two recessive informational suppressor genes were identified, supa4.1(+) and supa4.2(+) mapping <1.0 and 12.0 units from acu-1 respectively. Comparison of growth of suppressed mutants on selective (acetate) and non-selective (glucose) media indicated that supa4.1(+) was more efficient than supa4.2(+) but both restored an identically temperature sensitive enzyme function. Measurement of enzyme activity in cell free extracts showed that supa4.1(+) restored 29% wild type enzyme activity whereas supa4.2(+) restored 42%. Km for acetate of suppressed enzymes was indistinguishable from wild type but both had an identical but much reduced half life at 45 °C compared with wild type. These observations are consistent with supa4.1(+) and supa4.2(+) being tRNA suppressors derived by mutation in two genes encoding isoaccepting tRNAs.
Authors:
K H Vousden; L A Casselton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current genetics     Volume:  7     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  2013-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-5     Citation Subset:  -    
Affiliation:
School of Biological Sciences, Queen Mary College, Mile End Road, El 4NS, London, England.
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