Document Detail


Suppression of the SOS-inducing activity of Trp-P-1 and aflatoxin B1 by meso-dihydroguaiaretic acid from Machilus thunbergii in the Salmonella typhimurium TA1535/pSK1002 umu test.
MedLine Citation:
PMID:  9720227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The methanol extract from Machilus thunbergii showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires liver metabolizing enzymes. The methanol extract from M. thunbergii was successively re-extracted with chloroform, butanol and water. A suppressive compound in the chloroform extract fraction was isolated by SiO2 column chromatography and identified as meso-dihydroguaiaretic acid by GC-MS, and 1H- and 13C-NMR spectroscopy. Meso-dihydroguaiaretic acid inhibited of the SOS-inducing activity of Trp-P-1 in the umu test. Gene expression was suppressed by 62% at less than 0.18 mumol/ml, the ID50 value being 0.08 mumol/ml. Compound 1 was also assayed with aflatoxin B1 (AfB1) and showed a suppressive effect.
Authors:
M Miyazawa; Y Okuno; K Oshiro; H Kasahara; H Shimamura; S Nakamura; H Kameoka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  62     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-09-30     Completed Date:  1998-09-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1425-7     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University Kowakae, Osaka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aflatoxin B1 / toxicity*
Carbolines / toxicity*
Chloroform
DNA Damage / drug effects
Gas Chromatography-Mass Spectrometry
Guaiacol / analogs & derivatives*,  isolation & purification,  pharmacology
Lauraceae / chemistry*
Lignans / isolation & purification,  pharmacology*
Magnetic Resonance Spectroscopy
Mutagens / toxicity*
Plant Extracts / pharmacology
SOS Response (Genetics) / drug effects*
Salmonella typhimurium / drug effects*,  genetics
Chemical
Reg. No./Substance:
0/Carbolines; 0/Lignans; 0/Mutagens; 0/Plant Extracts; 1162-65-8/Aflatoxin B1; 36469-60-0/dihydroguaiaretic acid; 62450-06-0/Trp-P-1; 67-66-3/Chloroform; 90-05-1/Guaiacol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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