| Suppression of Par-4 protects human renal proximal tubule cells from apoptosis induced by oxidative stress. | |
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MedLine Citation:
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PMID: 20814219 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND: Oxidative stress is an important inducer of cell apoptosis and plays a key role in the development of renal inflammation. The prostate apoptosis response factor-4 (Par-4) gene was originally identified in prostate cells undergoing apoptosis. Subsequently, Par-4 was found to possess potent pro-apoptotic activity in various cellular systems. However, it remains unclear whether Par-4 is involved in oxidant injury of renal tubular epithelial cells. AIMS: To determine the role of Par-4 in renal proximal tubular cell apoptosis induced by oxidative stress. METHODS: Par-4 gene expression was silenced by small interfering RNA. Renal proximal tubular cells were then exposed to hydrogen peroxide and the effect of Par-4 silencing on apoptosis and expression of phosphorylated Akt and vascular endothelial growth factor was determined. RESULTS: Hydrogen peroxide induced apoptosis and increased Par-4 expression in human renal proximal tubular epithelial cells. Par-4 silencing significantly protected renal proximal tubular cells from apoptosis via activating the PI3K/Akt signaling pathway as Akt phosphorylation was enhanced. Par-4 silencing also ameliorated the downregulation of vascular endothelial growth factor expression induced by oxidative stress. CONCLUSION: Par-4 gene silencing resulted in PI3K/Akt signaling-dependent inhibition of renal proximal tubular cell apoptosis following oxidative stress. |
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Authors:
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Bin Sun; Chao Lu; Guo-Ping Zhou; Chang-Ying Xing |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nephron. Experimental nephrology Volume: 117 ISSN: 1660-2129 ISO Abbreviation: Nephron Exp. Nephrol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-04-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101159770 Medline TA: Nephron Exp Nephrol Country: Switzerland |
Other Details:
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Languages: eng Pagination: e53-61 Citation Subset: IM |
Affiliation:
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Division of Nephrology, Department of Internal Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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