| Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice. | |
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MedLine Citation:
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PMID: 20507922 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study tested the hypothesis that resveratrol supplementation would lower oxidative stress in exercised muscles of aged mice. Young (3 months) and aged (27 months) C57BL/6 mice received a control or a 0.05% trans-resveratrol-supplemented diet for 10 days. After 7 days of dietary intervention, 20 maximal electrically evoked isometric contractions were obtained from the plantar flexors of one limb in anesthetized mice. Exercise was conducted for three consecutive days. Resveratrol supplementation blunted the exercise-induced increase in xanthine oxidase activity in muscles from young (25%) and aged (53%) mice. Resveratrol lowered H(2)O(2) levels in control (13%) and exercised (38%) muscles from aged animals, reduced Nox4 protein in both control and exercised muscles of young (30%) and aged mice (40%), and increased the ratio of reduced glutathione to oxidized glutathione in exercised muscles from young (38%) and aged (135%) mice. Resveratrol prevented the increase in lipid oxidation, increased catalase activity, and increased MnSOD activity in exercised muscles from aged mice. These data show that dietary resveratrol suppresses muscle indicators of oxidative stress in response to isometric contractions in aged mice. |
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Authors:
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Michael J Ryan; Janna R Jackson; Yanlei Hao; Courtney L Williamson; Erinne R Dabkowski; John M Hollander; Stephen E Alway |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-05-27 |
Journal Detail:
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Title: The journals of gerontology. Series A, Biological sciences and medical sciences Volume: 65 ISSN: 1758-535X ISO Abbreviation: J. Gerontol. A Biol. Sci. Med. Sci. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2010-08-04 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 9502837 Medline TA: J Gerontol A Biol Sci Med Sci Country: United States |
Other Details:
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Languages: eng Pagination: 815-31 Citation Subset: AIM; IM |
Affiliation:
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Division of Exercise Physiology, West Virginia University School of Medicine, PO Box 9227, Morgantown, WV 26506, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Animals Body Weight Citrate (si)-Synthase / metabolism Eating Glutathione / metabolism Hydrogen Peroxide / metabolism Lipid Peroxidation Mice Mice, Inbred C57BL Muscle Contraction / drug effects* Muscle Fatigue / drug effects Muscle, Skeletal / drug effects*, metabolism NADPH Oxidase / metabolism Oxidative Stress / drug effects* Stilbenes / pharmacology* Superoxide Dismutase / metabolism Xanthine Oxidase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01AG021530/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Stilbenes; 501-36-0/resveratrol; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase; EC 1.6.3.1/NADPH Oxidase; EC 2.3.3.1/Citrate (si)-Synthase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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