Document Detail


Suppression of mitochondrial electron transport chain function in the hypoxic human placenta: a role for miRNA-210 and protein synthesis inhibition.
MedLine Citation:
PMID:  23383105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fetal growth is critically dependent on energy metabolism in the placenta, which drives active exchange of nutrients. Placental oxygen levels are therefore vital, and chronic hypoxia during pregnancy impairs fetal growth. Here we tested the hypothesis that placental hypoxia alters mitochondrial electron transport chain (ETS) function, and sought to identify underlying mechanisms. We cultured human placental cells under different oxygen concentrations. Mitochondrial respiration was measured, alongside levels of ETS complexes. Additionally, we studied placentas from sea-level and high-altitude pregnancies. After 4 d at 1% O₂ (1.01 KPa), complex I-supported respiration was 57% and 37% lower, in trophoblast-like JEG3 cells and fibroblasts, respectively, compared with controls cultured at 21% O₂ (21.24 KPa); complex IV-supported respiration was 22% and 30% lower. Correspondingly, complex I levels were 45% lower in placentas from high-altitude pregnancies than those from sea-level pregnancies. Expression of HIF-responsive microRNA-210 was increased in hypoxic fibroblasts and high-altitude placentas, whilst expression of its targets, iron-sulfur cluster scaffold (ISCU) and cytochrome c oxidase assembly protein (COX10), decreased. Moreover, protein synthesis inhibition, a feature of the high-altitude placenta, also suppressed ETS complex protein levels. Our results demonstrate that mitochondrial function is altered in hypoxic human placentas, with specific suppression of complexes I and IV compromising energy metabolism and potentially contributing to impaired fetal growth.
Authors:
Francesca Colleoni; Nisha Padmanabhan; Hong-Wa Yung; Erica D Watson; Irene Cetin; Martha C Tissot van Patot; Graham J Burton; Andrew J Murray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-30
Journal Detail:
Title:  PloS one     Volume:  8     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2013  
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-22     Revised Date:  2014-10-31    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e55194     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkyl and Aryl Transferases / metabolism
Altitude
Analysis of Variance
Anoxia / metabolism,  physiopathology*
Blotting, Western
Cell Culture Techniques
Cell Line
Electron Transport Chain Complex Proteins / antagonists & inhibitors*
Electron Transport Complex IV / metabolism
Energy Metabolism / physiology*
Female
Humans
Iron-Sulfur Proteins / metabolism
Membrane Proteins / metabolism
MicroRNAs / metabolism*,  pharmacology
Oxygen / metabolism
Placenta / physiopathology*
Pregnancy
Real-Time Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
084804/2/08/Z//Wellcome Trust; //Medical Research Council
Chemical
Reg. No./Substance:
0/Electron Transport Chain Complex Proteins; 0/ISCU protein, human; 0/Iron-Sulfur Proteins; 0/MIRN210 microRNA, human; 0/Membrane Proteins; 0/MicroRNAs; EC 1.9.3.1/COX10 protein, human; EC 1.9.3.1/Electron Transport Complex IV; EC 2.5.-/Alkyl and Aryl Transferases; S88TT14065/Oxygen
Comments/Corrections
Erratum In:
PLoS One. 2014;9(4):e93245

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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