Document Detail

Suppression of Helicobacter pylori-induced interleukin-8 production in vitro and within the gastric mucosa by a live Lactobacillus strain.
MedLine Citation:
PMID:  16911683     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Accumulating evidence indicates that interleukin-8 (IL-8) plays a major role in the mucosal inflammation caused by Helicobacter pylori infection. The purpose of the present study was to examine whether Lactobacillus gasseri OLL2716 (LG21) can inhibit the H. pylori-induced production of IL-8. METHODS: A coculture system including MKN45 cells, H. pylori, and LG21 was established for an in vitro analysis. Biopsy specimens were obtained from H. pylori-infected human subjects consisting of 19 men and six women. RESULTS: When LG21 was 1/100 less than H. pylori in a coculture system, LG21 significantly suppressed both the IL-8 mRNA and protein generation in the coculture. Live, but not heat- or UV-treated LG21, could exert the suppressive effect. However, this amount of LG21 could not suppress either the adhesion of H. pylori to the cell surface or the IL-8 production by tumor necrosis factor-alpha, which induces IL-8 generation through the activation of the transcription. These results thus suggest that LG21 suppresses an event leading to IL-8 production, which is specific for H. pylori-induced IL-8 generation, and this event is located upstream from the IL-8 transcription but downstream from the adhesion. The measurement of the IL-8 level using gastric biopsy specimens from H. pylori-infected subjects demonstrated that LG21 also suppresses the production of IL-8 in the gastric mucosa. CONCLUSIONS: Live LG21 were found to suppress H. pylori-induced IL-8 production in both a gastric cell line and within gastric mucosa.
Akira Tamura; Hideshi Kumai; Noboru Nakamichi; Toshirou Sugiyama; Ryozo Deguchi; Atsusi Takagi; Yasuhiro Koga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  21     ISSN:  0815-9319     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-16     Completed Date:  2007-05-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1399-406     Citation Subset:  IM    
Laboratory for Infectious Diseases, Tokai University Medical School, Bohseidai, Isehara, Japan.
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MeSH Terms
Cell Adhesion / physiology
Cell Line
Coculture Techniques
Gastric Mucosa* / immunology,  microbiology
Helicobacter Infections / immunology
Helicobacter pylori / immunology*
Interleukin-8 / genetics,  immunology*
Lactobacillus / metabolism*
Middle Aged
NF-kappa B / metabolism
Signal Transduction / physiology
Reg. No./Substance:
0/Interleukin-8; 0/NF-kappa B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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