| Suppression of H-ras-mediated transformation in NIH3T3 cells by a ras ribozyme. | |
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MedLine Citation:
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PMID: 7945447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Murine NIH3T3 cells were used to study the effect of ribozymes on H-ras-mediated transformation. Parental 3T3 cells were transfected with the activated H-ras gene. H-ras-transformed cells had altered morphology and increased colony formation in soft agar in contrast to untransfected 3T3 cells. A hammerhead ribozyme (site-specific ribonuclease) designed to cleave codon 12 (GUC) of the activated H-ras RNA was expressed in transformed cells. 3T3 clones expressing the ras ribozyme displayed decreased expression of activated H-ras RNA. The ras ribozyme reversed the transformed phenotype to resemble that of untransfected 3T3 cells. Furthermore, 3T3 cells containing the ras ribozyme were shown to suppress transformation when they were subsequently transfected with activated H-ras. Insertion of a mutant ribozyme largely devoid of cleaving capacity into H-ras-transformed cells resulted in smaller reductions in H-ras gene expression and colony formation in soft agar when compared with the ras ribozyme. Finally, the ras ribozyme alone did not perturb normal 3T3 cell growth. This study suggests the possible utility of anti-oncogene ribozymes as suppressors of tumor cell growth as well as inhibitors of cellular transformation. |
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Authors:
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T Funato; T Shitara; T Tone; L Jiao; M Kashani-Sabet; K J Scanlon |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical pharmacology Volume: 48 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-11-23 Completed Date: 1994-11-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1471-5 Citation Subset: IM |
Affiliation:
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Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA 91010. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Base Sequence Cell Transformation, Neoplastic / genetics* Genes, ras* Mice Molecular Sequence Data Mutation Oncogenes Plasmids RNA, Catalytic / genetics* Suppression, Genetic Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA 50618/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Catalytic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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