Document Detail

Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid.
MedLine Citation:
PMID:  12570057     Owner:  NLM     Status:  MEDLINE    
We examined the expression of early growth response-1 (Egr-1) gene in human oral squamous carcinoma cell lines SCCKN and SCC-25 cells and human osteoblastic cell lines Saos-2 and MG63 cells treated with okadaic acid, a potent inhibitor of protein phosphatases type 1 and type 2A. Western blot analysis revealed that Egr-1 was strongly expressed in SCCKN cells and that okadaic acid decreased the expression of Egr-1 protein in these cells. However, Egr-1 was expressed at lower levels in SCC-25, Saos-2, and MG63 cells and transiently increased with the okadaic acid treatment. Suppression of Egr-1 protein expression in okadaic acid-treated SCCKN cells stemmed from the suppression of the Egr-1 mRNA level, as determined by the RT-RCR method. Formaldehyde-fixed and alcohol-permeabilized cultured SCCKN cells were reacted with the anti-Egr-1 antibody using immunohistochemical methods. Intense fluorescence was observed in the nuclei of the control SCCKN cells interacted with anti-Egr-1 antibody. However, only a weak reaction was observed in the nuclei in SCCKN cells treated with okadaic acid. A gel mobility shift assay showed that treatment of SCCKN cells with okadaic acid suppressed Egr-1 binding to the DIG-labeled Egr-1 consensus oligonucleotide probe. The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level.
H Okamura; H Morimoto; M Fujita; F Nasu; E Sasakia; T Haneji
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral oncology     Volume:  38     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-02-06     Completed Date:  2003-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  779-84     Citation Subset:  IM    
Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3-18-15, Kuramoto, Tokushima 770-8504, Japan.
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MeSH Terms
Carcinogens / therapeutic use*
Carcinoma, Squamous Cell / drug therapy*,  genetics,  metabolism
DNA-Binding Proteins / genetics,  metabolism*
Early Growth Response Protein 1
Electrophoresis, Agar Gel / methods
Gene Expression Regulation
Immediate-Early Proteins*
Immunohistochemistry / methods
Mouth Neoplasms / drug therapy*,  genetics,  metabolism
Neoplasm Proteins / genetics,  metabolism*
Okadaic Acid / therapeutic use*
Reverse Transcriptase Polymerase Chain Reaction / methods
Suppression, Genetic
Transcription Factors / genetics,  metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/Carcinogens; 0/DNA-Binding Proteins; 0/EGR1 protein, human; 0/Early Growth Response Protein 1; 0/Immediate-Early Proteins; 0/Neoplasm Proteins; 0/Transcription Factors; 78111-17-8/Okadaic Acid

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