Document Detail


Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid.
MedLine Citation:
PMID:  12570057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the expression of early growth response-1 (Egr-1) gene in human oral squamous carcinoma cell lines SCCKN and SCC-25 cells and human osteoblastic cell lines Saos-2 and MG63 cells treated with okadaic acid, a potent inhibitor of protein phosphatases type 1 and type 2A. Western blot analysis revealed that Egr-1 was strongly expressed in SCCKN cells and that okadaic acid decreased the expression of Egr-1 protein in these cells. However, Egr-1 was expressed at lower levels in SCC-25, Saos-2, and MG63 cells and transiently increased with the okadaic acid treatment. Suppression of Egr-1 protein expression in okadaic acid-treated SCCKN cells stemmed from the suppression of the Egr-1 mRNA level, as determined by the RT-RCR method. Formaldehyde-fixed and alcohol-permeabilized cultured SCCKN cells were reacted with the anti-Egr-1 antibody using immunohistochemical methods. Intense fluorescence was observed in the nuclei of the control SCCKN cells interacted with anti-Egr-1 antibody. However, only a weak reaction was observed in the nuclei in SCCKN cells treated with okadaic acid. A gel mobility shift assay showed that treatment of SCCKN cells with okadaic acid suppressed Egr-1 binding to the DIG-labeled Egr-1 consensus oligonucleotide probe. The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level.
Authors:
H Okamura; H Morimoto; M Fujita; F Nasu; E Sasakia; T Haneji
Related Documents :
19995427 - Expression of hiwi in human esophageal squamous cell carcinoma is significantly associa...
12536197 - Antitumor effect of secreted flt3-ligand can act at distant tumor sites in a murine mod...
9054437 - The redistribution of cortactin into cell-matrix contact sites in human carcinoma cells...
17699797 - Syk tyrosine kinase is linked to cell motility and progression in squamous cell carcino...
24324357 - Insights into the hallmarks of human nucleus pulposus cells with particular reference t...
25477927 - Whole cells in enantioselective reduction of benzyl acetoacetate.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral oncology     Volume:  38     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-02-06     Completed Date:  2003-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  779-84     Citation Subset:  IM    
Affiliation:
Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3-18-15, Kuramoto, Tokushima 770-8504, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carcinogens / therapeutic use*
Carcinoma, Squamous Cell / drug therapy*,  genetics,  metabolism
DNA-Binding Proteins / genetics,  metabolism*
Down-Regulation
Early Growth Response Protein 1
Electrophoresis, Agar Gel / methods
Gene Expression Regulation
Humans
Immediate-Early Proteins*
Immunohistochemistry / methods
Mouth Neoplasms / drug therapy*,  genetics,  metabolism
Neoplasm Proteins / genetics,  metabolism*
Okadaic Acid / therapeutic use*
Reverse Transcriptase Polymerase Chain Reaction / methods
Suppression, Genetic
Transcription Factors / genetics,  metabolism*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Carcinogens; 0/DNA-Binding Proteins; 0/EGR1 protein, human; 0/Early Growth Response Protein 1; 0/Immediate-Early Proteins; 0/Neoplasm Proteins; 0/Transcription Factors; 78111-17-8/Okadaic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of beta-catenin in rat oral epithelial dysplasia induced by 4-nitroquinoline 1-oxide.
Next Document:  Osteosarcoma arising in Paget's disease of the mandible.