Document Detail

Suppression of Drosophila cellular immunity by directed expression of the ExoS toxin GAP domain of Pseudomonas aeruginosa.
MedLine Citation:
PMID:  15888083     Owner:  NLM     Status:  MEDLINE    
We show here that transgenic Drosophila can be used to decipher the effect of a bacterial toxin on innate immunity and demonstrate the contribution of blood cells in fly resistance to bacterial infection. ExoS is a Pseudomonas aeruginosa exotoxin directly translocated into the host cell cytoplasm through the type III secretion system found in many Gram-negative bacteria. It contains a N-terminal GTPase activating (GAP) domain that prevents cytoskeleton reorganization by Rho family of GTPases in cell culture. Directed expression of the ExoS GAP domain (ExoSGAP) during fly eye morphogenesis inhibited Rac1-, Cdc42- and Rho-dependent signalling, demonstrating for the first time its activity on RhoGTPases in a whole organism. We further showed that fly resistance to P. aeruginosa infections was altered when ExoSGAP was expressed either ubiquitously or in haemocytes, but not when expressed into the fat body, the major source of NF-(kappa)B-dependent anti-microbial peptide synthesis. Fly sensitivity to infection was also observed with Gram-positive Staphylococcus aureus strain and was associated to a reduced phagocytosis capacity of ExoSGAP-expressing haemocytes. Our results highlight the major contribution of cellular immunity during the first hours after Drosophila infection by P. aeruginosa, an opportunist pathogen affecting patients with pathologies associated to a reduced leukocyte number.
Amelie Avet-Rochex; Evelyne Bergeret; Ina Attree; Marie Meister; Marie-Odile Fauvarque
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular microbiology     Volume:  7     ISSN:  1462-5814     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-12     Completed Date:  2005-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  799-810     Citation Subset:  IM    
CEA-Grenoble, Département de Réponse et Dynamique Cellulaires, UMR5092 CEA/CNRS/UJF, 17, rue des Martyrs 38054 Grenoble cedex 9, France.
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MeSH Terms
ADP Ribose Transferases / biosynthesis*,  genetics
Animals, Genetically Modified
Bacterial Toxins / biosynthesis*,  genetics
Drosophila melanogaster / growth & development,  immunology*,  metabolism*,  microbiology
Eye / growth & development
Fat Body / metabolism
GTPase-Activating Proteins / genetics
Hemocytes / metabolism
Microscopy, Electron, Scanning
Protein Structure, Tertiary
Pseudomonas aeruginosa / genetics*,  physiology
Signal Transduction
Staphylococcus aureus / pathogenicity
rho GTP-Binding Proteins / metabolism
Reg. No./Substance:
0/Bacterial Toxins; 0/GTPase-Activating Proteins; EC 2.4.2.-/ADP Ribose Transferases; EC S; EC GTP-Binding Proteins

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