Document Detail

Suppressing unspecific cell uptake for targeted delivery using hydroxyethyl starch nanocapsules.
MedLine Citation:
PMID:  22844871     Owner:  NLM     Status:  Publisher    
Abstract Synthesizing nanocarriers with stealth properties and delivering a "payload" to the particular organ still remains a big challenge but is the prime prerequisite for any in vivo application. As non-toxic alternative to the modification by poly(ethylene glycol) PEG, we describe the synthesis of crosslinked hydroxyethyl starch (HES, Mw 200,000 g/mol) nanocapsules in the size range of 170-300 nm which do not show non-specific uptake into cells. The specific uptake was shown by coupling a folic acid conjugate as model targeting agent onto the surface of the nanocapsules, since folic acid has a high affinity to a variety of human carcinoma cell lines overexpressing the folate receptor on the surface. The covalent binding of the folic acid conjugate onto HES capsules was confirmed by FTIR and NMR spectroscopy. The coupling efficiency was determined using fluorescence spectroscopy. The specific cellular uptake of the HES nanocapsules after folic acid coupling into folate-receptor presenting cells was studied by confocal laser scanning microscopy (CLSM) and flow cytometry.
Grit Baier; Daniela Baumann; Joerg Max Siebert; Anna Musyanovych; Volker Mailaender; Katharina Landfester
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-30
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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