Document Detail

Supporting cells eliminate dying sensory hair cells to maintain epithelial integrity in the avian inner ear.
MedLine Citation:
PMID:  20844149     Owner:  NLM     Status:  MEDLINE    
Epithelial homeostasis is essential for sensory transduction in the auditory and vestibular organs of the inner ear, but how it is maintained during trauma is poorly understood. To examine potential repair mechanisms, we expressed β-actin-enhanced green fluorescent protein (EGFP) in the chick inner ear and used live-cell imaging to study how sensory epithelia responded during aminoglycoside-induced hair cell trauma. We found that glial-like supporting cells used two independent mechanisms to rapidly eliminate dying hair cells. Supporting cells assembled an actin cable at the luminal surface that extended around the pericuticular junction and constricted to excise the stereocilia bundle and cuticular plate from the hair cell soma. Hair bundle excision could occur within 3 min of actin-cable formation. After bundle excision, typically with a delay of up to 2-3 h, supporting cells engulfed and phagocytosed the remaining bundle-less hair cell. Dual-channel recordings with β-actin-EGFP and vital dyes revealed phagocytosis was concurrent with loss of hair cell integrity. We conclude that supporting cells repaired the epithelial barrier before hair cell plasmalemmal integrity was lost and that supporting cell activity was closely linked to hair cell death. Treatment with the Rho-kinase inhibitor Y-27632 did not prevent bundle excision but prolonged phagocytic engulfment and resulted in hair cell corpses accumulating within the epithelium. Our data show that supporting cells not only maintain epithelial integrity during trauma but suggest they may also be an integral part of the hair cell death process itself.
Jonathan E Bird; Nicolas Daudet; Mark E Warchol; Jonathan E Gale
Related Documents :
19003249 - Cultivation of hek 293 cell line and production of a member of the superfamily of g-pro...
6783729 - Ultrastructural aspect of size dependent regulation of surface pattern of complex cilia...
18029379 - The autoregulation of nodulation mechanism is related to leaf development.
19158839 - Profiling the response of human hair follicles to ultraviolet radiation.
19064919 - Cells of adult brain germinal zone have properties akin to hair cells and can be used t...
19397899 - New ectopic vestibular hair cell-like cells induced by math1 gene transfer in postnatal...
2358049 - Behavioral correlates of theta-on and theta-off cells recorded from hippocampal formati...
16323149 - Cell volume increase in murine mc3t3-e1 pre-osteoblasts attaching onto biocompatible ta...
12706369 - A proteome study of secreted prostatic factors affecting osteoblastic activity: identif...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  30     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-16     Completed Date:  2010-10-25     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12545-56     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actin Cytoskeleton / physiology,  ultrastructure
Aminoglycosides / toxicity
Cell Communication / physiology
Cell Death / drug effects,  physiology
Cell Membrane / physiology,  ultrastructure
Cilia / physiology,  ultrastructure
Cochlea / cytology,  growth & development
Epithelial Cells / cytology*,  physiology*
Green Fluorescent Proteins / genetics,  metabolism
Hair Cells, Auditory / cytology,  physiology*
Homeostasis / physiology
Labyrinth Supporting Cells / cytology*,  physiology*
Neurotoxins / toxicity
Organ Culture Techniques
Phagocytosis / drug effects,  physiology
Regeneration / physiology
Grant Support
R01 DC006283/DC/NIDCD NIH HHS; R01 DC006283-02/DC/NIDCD NIH HHS; R01DC006283/DC/NIDCD NIH HHS; //Medical Research Council
Reg. No./Substance:
0/Aminoglycosides; 0/Neurotoxins; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Pathogenic lysosomal depletion in Parkinson's disease.
Next Document:  PKCzeta regulates cell polarisation and proliferation restriction during mammary acinus formation.