| Supporting cells eliminate dying sensory hair cells to maintain epithelial integrity in the avian inner ear. | |
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MedLine Citation:
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PMID: 20844149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epithelial homeostasis is essential for sensory transduction in the auditory and vestibular organs of the inner ear, but how it is maintained during trauma is poorly understood. To examine potential repair mechanisms, we expressed β-actin-enhanced green fluorescent protein (EGFP) in the chick inner ear and used live-cell imaging to study how sensory epithelia responded during aminoglycoside-induced hair cell trauma. We found that glial-like supporting cells used two independent mechanisms to rapidly eliminate dying hair cells. Supporting cells assembled an actin cable at the luminal surface that extended around the pericuticular junction and constricted to excise the stereocilia bundle and cuticular plate from the hair cell soma. Hair bundle excision could occur within 3 min of actin-cable formation. After bundle excision, typically with a delay of up to 2-3 h, supporting cells engulfed and phagocytosed the remaining bundle-less hair cell. Dual-channel recordings with β-actin-EGFP and vital dyes revealed phagocytosis was concurrent with loss of hair cell integrity. We conclude that supporting cells repaired the epithelial barrier before hair cell plasmalemmal integrity was lost and that supporting cell activity was closely linked to hair cell death. Treatment with the Rho-kinase inhibitor Y-27632 did not prevent bundle excision but prolonged phagocytic engulfment and resulted in hair cell corpses accumulating within the epithelium. Our data show that supporting cells not only maintain epithelial integrity during trauma but suggest they may also be an integral part of the hair cell death process itself. |
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Authors:
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Jonathan E Bird; Nicolas Daudet; Mark E Warchol; Jonathan E Gale |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 30 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-16 Completed Date: 2010-10-25 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 12545-56 Citation Subset: IM |
Affiliation:
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UCL Ear Institute, University College London, London WC1X 8EE, United Kingdom. birdjo@nidcd.nih.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminoglycosides
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toxicity Animals Cell Communication / physiology Cell Death / drug effects, physiology Cell Membrane / physiology, ultrastructure Chickens Cilia / physiology, ultrastructure Cochlea / cytology, growth & development Epithelial Cells / cytology*, physiology* Green Fluorescent Proteins / genetics, metabolism Hair Cells, Auditory / cytology, physiology* Homeostasis / physiology Labyrinth Supporting Cells / cytology*, physiology* Microfilaments / physiology, ultrastructure Neurotoxins / toxicity Organ Culture Techniques Phagocytosis / drug effects, physiology Regeneration / physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DC006283-02/DC/NIDCD NIH HHS; R01DC006283/DC/NIDCD NIH HHS; //Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/Aminoglycosides; 0/Neurotoxins; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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