Document Detail


Supply of fatty acid is one limiting factor in the accumulation of triacylglycerol in developing embryos
MedLine Citation:
PMID:  10444089     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The metabolic factors that determine oil yield in seeds are still not well understood. To begin to examine the limits on triacylglycerol (TAG) production, developing Cuphea lanceolata, Ulmus carpinifolia, and Ulmus parvifolia embryos were incubated with factors whose availability might limit oil accumulation. The addition of glycerol or sucrose did not significantly influence the rate of TAG synthesis. However, the rate of (14)C-TAG synthesis upon addition of 2.1 mM (14)C-decanoic acid (10:0) was approximately four times higher than the in vivo rate of TAG accumulation in C. lanceolata and two times higher than the in vivo rate in U. carpinifolia and U. parvifolia. In C. lanceolata embryos, the highest rate of (14)C-TAG synthesis (14.3 nmol h(-1) embryo(-1)) was achieved with the addition of 3.6 mM decanoic acid. (14)C-Decanoic acid was incorporated equally well in all three acyl positions of TAG. The results suggest that C. lanceolata, U. carpinifolia, and U. parvifolia embryos have sufficient acyltransferase activities and glycerol-3-phosphate levels to support rates of TAG synthesis in excess of those found in vivo. Consequently, the amount of TAG synthesized in these oilseeds may be in part determined by the amount of fatty acid produced in plastids.
Authors:
Bao; Ohlrogge
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Plant physiology     Volume:  120     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-08-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  Eng     Pagination:  1057-62     Citation Subset:  -    
Affiliation:
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824, USA.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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