Document Detail

Supply of fatty acid is one limiting factor in the accumulation of triacylglycerol in developing embryos
MedLine Citation:
PMID:  10444089     Owner:  NLM     Status:  Publisher    
The metabolic factors that determine oil yield in seeds are still not well understood. To begin to examine the limits on triacylglycerol (TAG) production, developing Cuphea lanceolata, Ulmus carpinifolia, and Ulmus parvifolia embryos were incubated with factors whose availability might limit oil accumulation. The addition of glycerol or sucrose did not significantly influence the rate of TAG synthesis. However, the rate of (14)C-TAG synthesis upon addition of 2.1 mM (14)C-decanoic acid (10:0) was approximately four times higher than the in vivo rate of TAG accumulation in C. lanceolata and two times higher than the in vivo rate in U. carpinifolia and U. parvifolia. In C. lanceolata embryos, the highest rate of (14)C-TAG synthesis (14.3 nmol h(-1) embryo(-1)) was achieved with the addition of 3.6 mM decanoic acid. (14)C-Decanoic acid was incorporated equally well in all three acyl positions of TAG. The results suggest that C. lanceolata, U. carpinifolia, and U. parvifolia embryos have sufficient acyltransferase activities and glycerol-3-phosphate levels to support rates of TAG synthesis in excess of those found in vivo. Consequently, the amount of TAG synthesized in these oilseeds may be in part determined by the amount of fatty acid produced in plastids.
Bao; Ohlrogge
Related Documents :
10065359 - Ketoprofen suppository dosage forms: in vitro release and in vivo absorption studies in...
2388689 - Mould growth on black table olives and prevention by sorbic acid, methyl-eugenol and sp...
8649229 - Dietary modulation of phospholipid fatty acid composition and lipoxygenase products in ...
17805849 - Therans-3-enoic acids ofaster alpinus andarctium minus seed oils.
17805679 - Gas-liquid chromatographic analysis of cyclopropene fatty acids.
12405779 - Production in large quantities of highly purified hydroxytyrosol from liquid-solid wast...
1153189 - Amino acids in carbonaceous chondrites.
7619399 - Development of metabolically engineered saccharomyces cerevisiae cells for the producti...
2981999 - Intestinal absorption and lipoprotein transport of (omega-3) eicosapentaenoic acid.
Publication Detail:
Journal Detail:
Title:  Plant physiology     Volume:  120     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-08-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  Eng     Pagination:  1057-62     Citation Subset:  -    
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Functional characterization and expression analysis of the amino acid permease RcAAP3 from castor be...
Next Document:  Accumulation of soybean glycinin and its assembly with the glutelins in rice