Document Detail


Supplemental palmitoleic (C16:1 cis-9) acid reduces lipogenesis and desaturation in bovine adipocyte cultures.
MedLine Citation:
PMID:  22851248     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Our objective was to determine if palmitoleic (C16:1 cis-9) acid supplementation to primary bovine adipocytes regulates lipogenic gene expression and rates of lipogenesis. Stromal vascular cells were isolated from subcutaneous and intermuscular fat, propagated, and frozen for use in this study. Cells were passaged 4 times, allowed to reach confluence, held for 2 d, and then differentiated with a standard hormone cocktail (d 0). At d 2, secondary differentiation media containing 1 of 4 levels of palmitoleic acid (0, 50, 150, or 300 μM) was added for 10 d. Cells were harvested on d 6 and 12 to assess fatty acid levels and gene expression. In addition, (13)C2 and (13)C18:0 stable isotopes were added on d 6 to measure lipogenesis and desaturase activity, respectively. Levels of C16:1 and total fatty acids increased (P < 0.05) linearly in response to palmitoleic acid supplement. Levels of C18:1 cis-11 and C20:1 cis-13 also increased (P < 0.01) in response to supplementation suggesting elongation of palmitoleic acid in vitro. Levels of C16:1, C18:1 cis-11, and total fatty acids were also greater (P < 0.05) at d 12 compared to d 6. In contrast, C16:0, C18:0, and C18:1 cis-9 levels decreased (P < 0.05) in response to palmitoleic acid supplementation and were not affected (P > 0.05) by harvest day. The ratio of C18:1 cis-9/C18:0 and fractional synthetic rate (FSR) of desaturation decreased (P < 0.05) in response to increasing palmitoleic acid supplementation. In addition, FSR of lipogenesis was reduced (P < 0.05) in palmitoleic acid-treated cells. Messenger RNA levels as determined by real time quantitative PCR for stearoyl-CoA desaturase 1 (SCD1), fatty acid synthase (FASN), and elongase protein 6 (ELOVL6) genes were reduced (P < 0.05) by palmitoleic acid supplementation. Expression of a β-oxidation gene, carnitine palmitoyltransferase 1A (CPT1A), was up-regulated (P < 0.05) with palmitoleic acid supplementation in a dose-responsive manner. Supplementation of palmitoleic acid to bovine adipocytes results in increased incorporation of this fatty acid and its elongation products into the adipocyte, which down-regulates SCD1, FASN, and ELOVL6 to decrease lipogenesis and up-regulates CPT1A, potentially increasing β-oxidation. These results suggest that palmitoleic acid, an end-product of desaturation, can act as a regulator of lipogenesis, desaturation, and β-oxidation in bovine adipocytes.
Authors:
T A Burns; S K Duckett; S L Pratt; T C Jenkins
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-31
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Clemson University, Clemson, SC 29634-0311.
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