Document Detail

Superposition of an incoherent magnetic field inhibited EGF receptor clustering and phosphorylation induced by a 1.8-GHz pulse-modulated radiofrequency radiation.
MedLine Citation:
PMID:  23210629     Owner:  NLM     Status:  Publisher    
Abstract Purpose: The present study was conducted to investigate the effect of a temporally incoherent ('noise') magnetic field (MF) on radiofrequency radiation (RFR)-induced epidermal growth factor (EGF) receptor clustering and phosporylation in cultured cells. Materials and methods: Human amniotic epithelial (FL) cells were exposed for 15 min to either a 1.8-GHz RFR (modulated at 217 Hz), a 2 μT incoherent MF, or concurrently to the RFR and incoherent MF. Epidermal growth factor treatment severed as the positive control. Epidermal growth factor receptor clustering on cellular membrane surface was analyzed using confocal microscopy after indirect immunofluorescence staining, and phosphorylation of EGF receptors was measured by western blot technology. Results: Exposure of FL cells to the 1.8-GHz RFR at SAR (specific absorption rate) of 0.5, 1.0, 2.0, or 4.0 W/kg for 15 min induced EGF receptor clustering and enhanced phosphorylation on tyrosine-1173 residue, whereas exposure to RFR at SAR of 0.1 W/kg for 15 min did not significantly cause these effects. Exposure to a 2 μT incoherent MF for 15 min did not significantly affect clustering and phosphorylation of EGF receptor in FL cells. When superimposed, the incoherent MF completely inhibited EGF receptor clustering and phosphorylation induced by RFR at SAR of 0.5, 1.0, and 2.0 W/kg, but did not inhibit the effects induced at SAR of 4.0 W/kg. Conclusion: Based on the data of the experiment, it is suggested that membrane receptors could be one of the main targets by which RFR interacts with cells. An incoherent MF could block the interaction to a certain extent.
Wenjun Sun; Xiuying Shen; Dongbo Lu; Deqiang Lu; Huai Chiang
Related Documents :
24332889 - Altered receptor binding densities in experimental antiphospholipid syndrome despite on...
20087739 - Progression and treatment of her2-positive breast cancer.
23724059 - Ppar-alpha agonists as novel antiepileptic drugs: preclinical findings.
25086039 - Different egf receptor ligands show distinct kinetics and biased or partial agonism for...
24080429 - Contribution of the lateral lemniscus to the control of swallowing in decerebrate cats.
23197649 - Allosteric modulation of a chemogenetically modified g protein-coupled receptor.
8787039 - The combination of several antidepressants is not synergistic on the firing of lateral ...
3586649 - The antiglucocorticoid, cortexolone, fails to promote in vitro activation of cytoplasmi...
7588379 - Stimulation of cortisol production through angiotensin at2 receptors in bovine fascicul...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Convenient Method for the Synthesis of a Flexible Cyclic Polyamide for Selective Targeting of c-myb ...
Next Document:  Pharmacological Maintenance Treatments of Opiate Addiction.