Document Detail


Superparamagnetic iron oxide labeling of spinal cord neural stem cells genetically modified by nerve growth factor-beta.
MedLine Citation:
PMID:  19399412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study established superparamagnetic iron oxide (SPIO)-labeled nerve growth factor-beta (NGF-beta) gene-modified spinal cord-derived neural stem cells (NSCs). The E14 rat embryonic spinal cord-derived NSCs were isolated and cultured. The cells of the third passage were transfected with plasmid pcDNA3-hNGFbeta by using FuGENE HD transfection reagent. The expression of NGF-beta was measured by immunocytochemistry and Western blotting. The positive clones were selected, allowed to proliferate and then labeled with SPIO, which was mediated by FuGENE HD transfection reagent. Prussian blue staining and transmission electron microscopy (TEM) were used to identify the SPIO particles in the cells. The distinctive markers for stem cells (nestin), neuron (beta-III-tubulin), oligodendrocyte (CNPase) and astrocyte (GFAP) were employed to evaluate the differentiation ability of the labeled cells. The immunocytochemistry and western blotting showed that NGF-beta was expressed in spinal cord-derived NSCs. Prussian blue staining indicated that numerous blue-stained particles appeared in the cytoplasma of the labeled cells. TEM showed that SPIO particles were found in vacuolar structures of different sizes and the cytoplasma. The immunocytochemistry demonstrated that the labeled cells were nestin-positive. After differentiation, the cells expressed beta-III-tubulin, CNPase and GFAP. It was concluded that the SPIO-labeled NGF-beta gene-modified spinal cord-derived NSC were successfully established, which are multipotent and capable of self-renewal.
Authors:
Deqiang Lei; Hongyang Zhao; Xingli Deng; Ruen Liu; Fangcheng Zhang; Dongxiao Yao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-28
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  29     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2011-03-17     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  235-8     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ldqtz@163.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Dextrans / diagnostic use*
Embryo, Mammalian
Magnetic Resonance Imaging
Magnetics
Magnetite Nanoparticles / diagnostic use*
Nerve Growth Factor / genetics*,  pharmacology
Neural Stem Cells / cytology*
Rats
Spinal Cord / cytology*
Transfection*
Chemical
Reg. No./Substance:
0/Magnetite Nanoparticles; 0/ferumoxtran-10; 9004-54-0/Dextrans; 9061-61-4/Nerve Growth Factor; G6N3J05W84/ferumoxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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