Document Detail


Superoxide triggers an acid burst in Saccharomyces cerevisiae to condition the environment of glucose-starved cells.
MedLine Citation:
PMID:  23281478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although yeast cells grown in abundant glucose tend to acidify their extracellular environment, they raise the pH of the environment when starved for glucose or when grown strictly with non-fermentable carbon sources. Following prolonged periods in this alkaline phase, Saccharomyces cerevisiae cells will switch to producing acid. The mechanisms and rationale for this "acid burst" were unknown. Herein we provide strong evidence for the role of mitochondrial superoxide in initiating the acid burst. Yeast mutants lacking the mitochondrial matrix superoxide dismutase (SOD2) enzyme, but not the cytosolic Cu,Zn-SOD1 enzyme, exhibited marked acceleration in production of acid on non-fermentable carbon sources. Acid production is also dramatically enhanced by the superoxide-producing agent, paraquat. Conversely, the acid burst is eliminated by boosting cellular levels of Mn-antioxidant mimics of SOD. We demonstrate that the acid burst is dependent on the mitochondrial aldehyde dehydrogenase Ald4p. Our data are consistent with a model in which mitochondrial superoxide damage to Fe-S enzymes in the tricarboxylic acid (TCA) cycle leads to acetate buildup by Ald4p. The resultant expulsion of acetate into the extracellular environment can provide a new carbon source to glucose-starved cells and enhance growth of yeast. By triggering production of organic acids, mitochondrial superoxide has the potential to promote cell population growth under nutrient depravation stress.
Authors:
J Allen Baron; Kaitlin M Laws; Janice S Chen; Valeria C Culotta
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-31
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  288     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-18     Completed Date:  2013-04-29     Revised Date:  2014-02-18    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4557-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / chemistry
Acids / chemistry*
Aldehyde Dehydrogenase / metabolism
Antioxidants / metabolism
Carbon / chemistry
Cytosol / enzymology
Environment
Glucose / chemistry,  metabolism*
Hydrogen-Ion Concentration
Mitochondria / metabolism
Oxidation-Reduction
Oxidative Stress
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism
Superoxides / chemistry*,  metabolism
Tricarboxylic Acids / chemistry
Grant Support
ID/Acronym/Agency:
ES 08996/ES/NIEHS NIH HHS; GM 50016/GM/NIGMS NIH HHS; P30 ES003819/ES/NIEHS NIH HHS; R01 ES008996/ES/NIEHS NIH HHS; R37 GM050016/GM/NIGMS NIH HHS; T32CA009110/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Acids; 0/Antioxidants; 0/Saccharomyces cerevisiae Proteins; 0/Tricarboxylic Acids; 11062-77-4/Superoxides; 7440-44-0/Carbon; EC 1.2.1.3/Aldehyde Dehydrogenase; IY9XDZ35W2/Glucose; Q40Q9N063P/Acetic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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