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Superoxide-signaling in perivascular adipose tissue promotes age-related artery stiffness.
MedLine Citation:
PMID:  24341314     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We tested the hypothesis that superoxide signaling within aortic perivascular adipose tissue (PVAT) contributes to large elastic artery stiffening in old mice. Young (4-6 mo), old (26-28 mo), and old treated with TEMPOL, a superoxide scavenger (1mM in drinking water for 3 weeks), male C57BL6/N mice were studied. Compared with young, old had greater large artery stiffness assessed by aortic pulse wave velocity (aPWV, 436±9 vs. 344±5 cm/s) and intrinsic mechanical testing (3821±427 vs. 1925±271 kPa) (both P<0.05). TEMPOL treatment in old reversed both measures of arterial stiffness. Aortic PVAT superoxide production was greater in old (P<0.05 vs. Y), which was normalized with TEMPOL. Compared with young, old controls had greater pro-inflammatory proteins in PVAT conditioned media (P<0.05). Young recipient mice transplanted with PVAT from old compared with young donors for 8 weeks had greater aPWV (409±7 vs. 342±8 cm/s) and intrinsic mechanical properties (3197±647 vs. 1889±520 kPa) (both P<0.05), which was abolished with TEMPOL supplementation in old donors. Tissue-cultured aortic segments from old in the presence of PVAT had greater mechanical stiffening compared with old cultured in the absence of PVAT and old with PVAT and TEMPOL (both, P<0.05). In addition, PVAT-derived superoxide was associated with arterial wall hypertrophy and greater adventitial collagen I expression with aging that was attenuated by TEMPOL. Aging or TEMPOL treatment did not affect blood pressure. Our findings provide evidence for greater age-related superoxide production and pro-inflammatory proteins in PVAT, and directly link superoxide signaling in PVAT to large elastic artery stiffness. This article is protected by copyright. All rights reserved.
Authors:
Bradley S Fleenor; Jason S Eng; Amy L Sindler; Bryant T Pham; Jackson D Kloor; Douglas R Seals
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-17
Journal Detail:
Title:  Aging cell     Volume:  -     ISSN:  1474-9726     ISO Abbreviation:  Aging Cell     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101130839     Medline TA:  Aging Cell     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
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