| Superoxide plays critical roles in electrotaxis of fibrosarcoma cells via activation of ERK and reorganization of cytoskeleton. | |
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MedLine Citation:
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PMID: 22406317 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Direct current electric field (DCEF) induces directional migration in many cell types through activating intracellular signaling pathways. However, the mechanisms coupling the extracellular electric stimulation to the intracellular signals remain largely unknown. In the current study, we showed that DCEF directed migration of HT-1080 fibrosarcoma cells to the cathode, stimulated generation of hydrogen peroxide and superoxide through the activation of NADPH oxidase, induced anode-facing cytoskeleton polarization, and activated ERK signaling. Subsequent studies demonstrated that the electrotaxis of HT-1080 fibrosarcoma cells was abolished by NADPH oxidase inhibitor and over-expression of manganese superoxide dismutase (MnSOD), an enzyme that hydrolyzes superoxide. In contrast, over-expression of catalases, which hydrolyze hydrogen peroxide, did not affect electrotaxis. MnSOD over-expression also eliminated cytoskeleton polarization as well as the activation of AKT, ERKs, and p38. In contrast, under catalase over-expression, cytoskeleton still polarized and p38 activation was affected. Finally, we showed that inhibition of ERKs activation also abolished DCEF-induced directional migration and cytoskeleton polarization. Collectively, our study indicated that superoxide plays critical roles in DCEF-induced directional migration of fibrosarcoma cells, possibly through regulating the activation of ERKs. This study provides novel insights into the current understanding of DCEF-mediated cancer cell directional migration and metastasis. |
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Authors:
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Fei Li; Hui Wang; Li Li; Chuanshu Huang; Jiangkai Lin; Gang Zhu; Zhi Chen; Nan Wu; Hua Feng |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-7 |
Journal Detail:
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Title: Free radical biology & medicine Volume: - ISSN: 1873-4596 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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