| Superoxide flashes in single mitochondria. | |
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MedLine Citation:
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PMID: 18662543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In quiescent cells, mitochondria are the primary source of reactive oxygen species (ROS), which are generated by leakiness of the electron transport chain (ETC). High levels of ROS can trigger cell death, whereas lower levels drive diverse and important cellular functions. We show here by employing a newly developed mitochondrial matrix-targeted superoxide indicator, that individual mitochondria undergo spontaneous bursts of superoxide generation, termed "superoxide flashes." Superoxide flashes occur randomly in space and time, exhibit all-or-none properties, and provide a vital source of superoxide production across many different cell types. Individual flashes are triggered by transient openings of the mitochondrial permeability transition pore stimulating superoxide production by the ETC. Furthermore, we observe a flurry of superoxide flash activity during reoxygenation of cardiomyocytes after hypoxia, which is inhibited by the cardioprotective compound adenosine. We propose that superoxide flashes could serve as a valuable biomarker for a wide variety of oxidative stress-related diseases. |
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Authors:
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Wang Wang; Huaqiang Fang; Linda Groom; Aiwu Cheng; Wanrui Zhang; Jie Liu; Xianhua Wang; Kaitao Li; Peidong Han; Ming Zheng; Jinhu Yin; Weidong Wang; Mark P Mattson; Joseph P Y Kao; Edward G Lakatta; Shey-Shing Sheu; Kunfu Ouyang; Ju Chen; Robert T Dirksen; Heping Cheng |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Cell Volume: 134 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-29 Completed Date: 2008-08-06 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 279-90 Citation Subset: IM |
Affiliation:
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Laboratories of Cardiovascular Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. wangwang@umich.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Cell Hypoxia Cell Line, Tumor Cells, Cultured Humans Luminescent Proteins / metabolism Mitochondria / metabolism* Muscle Cells / metabolism Myocytes, Cardiac / metabolism Neurons / metabolism Oxidative Stress Reactive Oxygen Species / metabolism Superoxides / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AR44657/AR/NIAMS NIH HHS; HL-33333/HL/NHLBI NIH HHS; R01 AR044657-11/AR/NIAMS NIH HHS; R01 GM056481-02/GM/NIGMS NIH HHS; R01 HL033333-12/HL/NHLBI NIH HHS; R01 HL093671-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/Reactive Oxygen Species; 11062-77-4/Superoxides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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