Document Detail


Superoxide flashes in single mitochondria.
MedLine Citation:
PMID:  18662543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In quiescent cells, mitochondria are the primary source of reactive oxygen species (ROS), which are generated by leakiness of the electron transport chain (ETC). High levels of ROS can trigger cell death, whereas lower levels drive diverse and important cellular functions. We show here by employing a newly developed mitochondrial matrix-targeted superoxide indicator, that individual mitochondria undergo spontaneous bursts of superoxide generation, termed "superoxide flashes." Superoxide flashes occur randomly in space and time, exhibit all-or-none properties, and provide a vital source of superoxide production across many different cell types. Individual flashes are triggered by transient openings of the mitochondrial permeability transition pore stimulating superoxide production by the ETC. Furthermore, we observe a flurry of superoxide flash activity during reoxygenation of cardiomyocytes after hypoxia, which is inhibited by the cardioprotective compound adenosine. We propose that superoxide flashes could serve as a valuable biomarker for a wide variety of oxidative stress-related diseases.
Authors:
Wang Wang; Huaqiang Fang; Linda Groom; Aiwu Cheng; Wanrui Zhang; Jie Liu; Xianhua Wang; Kaitao Li; Peidong Han; Ming Zheng; Jinhu Yin; Weidong Wang; Mark P Mattson; Joseph P Y Kao; Edward G Lakatta; Shey-Shing Sheu; Kunfu Ouyang; Ju Chen; Robert T Dirksen; Heping Cheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Cell     Volume:  134     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-08-06     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  279-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Cell Hypoxia
Cell Line, Tumor
Cells, Cultured
Humans
Luminescent Proteins / metabolism
Mitochondria / metabolism*
Muscle Cells / metabolism
Myocytes, Cardiac / metabolism
Neurons / metabolism
Oxidative Stress
Reactive Oxygen Species / metabolism
Superoxides / metabolism*
Grant Support
ID/Acronym/Agency:
AR44657/AR/NIAMS NIH HHS; HL-33333/HL/NHLBI NIH HHS; R01 AR044657/AR/NIAMS NIH HHS; R01 AR044657-11/AR/NIAMS NIH HHS; R01 GM056481/GM/NIGMS NIH HHS; R01 GM056481-02/GM/NIGMS NIH HHS; R01 HL033333/HL/NHLBI NIH HHS; R01 HL033333-12/HL/NHLBI NIH HHS; R01 HL093671/HL/NHLBI NIH HHS; R01 HL093671-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Reactive Oxygen Species; 11062-77-4/Superoxides
Comments/Corrections

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