Document Detail


Superoxide dismutase reduces islet microvascular injury induced by streptozotocin in the rat.
MedLine Citation:
PMID:  9277392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intravenous administration of streptozotocin (STZ) leads to permanent diabetes mellitus in rats. We investigated the possible role of islet microcirculatory changes and free radical formation in this animal model. In vivo fluorescence microscopy was performed for 4 h after administration of STZ. Vascular permeability, capillary blood flow, and endothelial leukocyte adhesion were measured in endocrine and exocrine pancreatic tissue. The earliest microcirculatory event was an increase in vascular permeability in pancreatic islets, with a peak 1 h after STZ administration. The difference between islet and exocrine tissue light intensity was +15.8 +/- 5.6% at t = 60 min. Islet blood flow velocity significantly decreased after 3 h, whereas blood flow in the exocrine pancreas was not affected. Complete stasis of islet blood flow was observed only in rats receiving STZ. Neither increased leukocyte adhesion to islet vascular endothelium nor ischemia-reperfusion phenomena were observed. Prophylactic administration of the radical scavenger superoxide dismutase prevented STZ-induced damage to the islet microcirculation in the initial phase of this model. We conclude that STZ leads to severe microcirculatory disturbances within pancreatic islets in rats. Apparently, these changes are mediated at least in part by free oxygen radicals.
Authors:
M Enghofer; K H Usadel; O Beck; K Kusterer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-24     Completed Date:  1997-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E376-82     Citation Subset:  IM    
Affiliation:
Department of Medicine I, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / analysis
Blood Vessels / drug effects,  pathology
Capillaries / physiology
Capillary Permeability / drug effects
Celiac Artery / physiology,  ultrasonography
Cell Adhesion / drug effects
Female
Islets of Langerhans / blood supply*
Leukocytes / physiology
Microcirculation / drug effects
Rats
Rats, Sprague-Dawley
Regional Blood Flow / drug effects
Streptozocin / pharmacology*
Superoxide Dismutase / pharmacology*
Chemical
Reg. No./Substance:
0/Blood Glucose; 18883-66-4/Streptozocin; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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