| Supercritical fluid extraction of ecdysterone from the roots of Achyranthes bidentata BL. | |
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MedLine Citation:
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PMID: 18389519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ecdysterone has been found in a great many plants and animals and has some valuable pharmaceutical properties. The present study was conducted to investigate optimal conditions for the extraction of the compound by supercritical fluid extraction from the roots of Achyranthes bidentata BL. An orthogonal array design (OAD), OA(9)(3(4)), was employed as a chemometric method for optimization of the extraction of ecdysterone from the herbal medicine. Four parameters, namely, pressure and temperature of the supercritical fluid, the dynamic extraction time, and the flow rate of dimethyl sulfoxide, were studied and optimized by a three-level OAD. Determinations of the extracts were performed by high-performance liquid chromatography. The effects of the parameters were studied using analysis of variance. The results shown that the yield of ecdysterone could be influenced by the four parameters to a similar degree. The yield for DMSO-modified supercritical CO(2) was in the range from 0.65 to 1.03 mg/g under the selected conditions. In comparison with methanol-modified supercritical CO(2 )and Soxhlet extraction, a higher yield was obtained when DMSO-modified supercritical CO(2) was used. |
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Authors:
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Yizhe Zheng; Ben Liu; Mingjie Chen; Tao Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of separation science Volume: 31 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-12 Completed Date: 2008-08-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 1393-8 Citation Subset: IM |
Affiliation:
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Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Achyranthes
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metabolism* Analysis of Variance Carbon Dioxide / chemistry Chemistry Techniques, Analytical / methods Chromatography / methods Chromatography, High Pressure Liquid / methods Chromatography, Supercritical Fluid / methods* Ecdysterone / analysis, chemistry, isolation & purification* Models, Chemical Plant Extracts / analysis* Plant Roots / metabolism* Plants, Medicinal / metabolism Pressure Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 124-38-9/Carbon Dioxide; 5289-74-7/Ecdysterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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