| Supercritical fluid extraction and characterization of lipids from algae Scenedesmus obliquus. | |
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MedLine Citation:
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PMID: 11540100 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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Supercritical carbon dioxide (SC-CO2) extractions (with and without ethanol as an entrainer) were carried out to remove lipids and pigments from protein concentrate of green algae (Scenedesmus obliquus) cultivated under controlled conditions. The content and fatty acid composition of algal lipids using column, thin-layer (TLC) and gas-liquid chromatography (GLC) were determined. Absorption spectra of extracted fractions showed the predominance of chlorophyll A (lambda max at 410 nm). Single step supercritical carbon dioxide (SC-CO2) extraction resulted mostly in removal of neutral lipids and a part of glycolipids, but phospholipids were not extracted. Addition of ethanol to SC-CO2 increased the amount of glycolipids and phospholipids in the extract. TLC pattern of algal lipids showed that the main part of neutral lipids consisted of diglycerides, triglycerides, hydrocarbons, free sterols, and sterol esters. The glycolipids were mostly monogalactosyl diglyceride, digalactosyl diglyceride, esterified sterol glycoside, and sterol glycoside. In phospholipids, phosphatidyl choline, phosphatidyl glycerol, and phosphatidyl ethanolamine were the main compounds. Fatty acid composition patterns indicated the main fatty acids to be 16:0, 16:1, 16:2, 16:3, 16:4, 18:1, 18:2, and 18:3(a). Relatively high recovery of polyunsaturated fatty acids and essential fatty acids in supercritical fluid extracted algal lipids and proteins isolates were observed. |
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Authors:
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K J Choi; Z Nakhost; V J Krukonis; M Karel |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Food biotechnology Volume: 1 ISSN: 0890-5436 ISO Abbreviation: Food Biotechnol Publication Date: 1987 |
Date Detail:
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Created Date: 1997-10-10 Completed Date: 1997-10-10 Revised Date: 2007-08-01 |
Medline Journal Info:
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Nlm Unique ID: 8802223 Medline TA: Food Biotechnol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 263-81 Citation Subset: S |
Affiliation:
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Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Green
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chemistry* Carbon Dioxide Chlorophyll / analysis Chromatography Environment, Controlled Ethanol Fatty Acids / analysis, isolation & purification Lipids / analysis*, chemistry, isolation & purification* Plant Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipids; 0/Plant Proteins; 124-38-9/Carbon Dioxide; 1406-65-1/Chlorophyll; 64-17-5/Ethanol |
| Investigator | |
Investigator/Affiliation:
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M Karel / MA Inst Tech, Cambridge |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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