Document Detail

Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells.
MedLine Citation:
PMID:  19709579     Owner:  NLM     Status:  Publisher    
Physalis peruviana L. (PP) is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. In this study, our objectives were to examine the total flavonoid and phenol content of different PP extracts (aqueous: HWEPP; ethanolic: EEPP; supercritical carbon dioxide: SCEPP-0, SCEPP-4 and SCEPP-5) and their antiproliferative effects in human lung cancer H661 cells. Among all the extracts tested, results showed that SCEPP-5 possessed the highest total flavonoid (226.19+/-4.15mg/g) and phenol (100.82+/-6.25mg/g) contents. SCEPP-5 also demonstrated the most potent inhibitory effect on H661 cell proliferation. Using DNA ladder and flow cytometry analysis, SCEPP-5 effectively induced H661 cell apoptosis as demonstrated by the accumulation of Sub-G1 peak and fragmentation of DNA. SCEPP-5 not only induced cell cycle arrest at S phase, it also up-regulated the expression of pro-apoptotic protein (Bax) and down-regulated the inhibitor of apoptosis protein (IAP). Furthermore, the apoptotic induction in H661 cells was found to associate with an elevated p53 protein expression, cytochrome c release, caspase-3 activation and PARP cleavage. Taken together, these results conclude that SCEPP-5 induced cell cycle arrest at S phase, and its apoptotic induction could be mediated through the p53-dependent pathway and modification of Bax and XIAP proteins expression. The results have also provided important pharmacological backgrounds for the potential use of PP supercritical fluid extract as products for cancer prevention.
Shu-Jing Wu; Shun-Pang Chang; Doung-Liang Lin; Shyh-Shyan Wang; Fwu-Feuu Hou; Lean-Teik Ng
Related Documents :
9141529 - Lovastatin-induced apoptosis in prostate stromal cells.
11282109 - Induction of apoptosis by esculetin in human leukemia cells.
9474719 - Characterization of apoptosis in a motor neuron cell line.
11351279 - Green tea polyphenol epigallocatechin inhibits dna replication and consequently induces...
10536169 - Constitutive expression of heregulin induces apoptosis in an erbb-2 overexpressing brea...
11397559 - Triptolide induced cytotoxic effects on human promyelocytic leukemia, t cell lymphoma a...
21901159 - Serratia marcescens is able to survive and proliferate in autophagic-like vacuoles insi...
9811589 - A role for rhob in the delamination of neural crest cells from the dorsal neural tube.
1319319 - Intermediate filaments and steroidogenesis in adrenal y-1 cells: acrylamide stimulation...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2009-2-4
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-8-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Graduate Institute of Nutritional Health, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cytotoxicity and genotoxicity of superporous hydrogel containing interpenetrating polymer networks.
Next Document:  Gardenia jasminoides Ellis ethanol extract and its constituents reduce the risks of gastritis and re...