Document Detail


Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells.
MedLine Citation:
PMID:  19709579     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Physalis peruviana L. (PP) is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. In this study, our objectives were to examine the total flavonoid and phenol content of different PP extracts (aqueous: HWEPP; ethanolic: EEPP; supercritical carbon dioxide: SCEPP-0, SCEPP-4 and SCEPP-5) and their antiproliferative effects in human lung cancer H661 cells. Among all the extracts tested, results showed that SCEPP-5 possessed the highest total flavonoid (226.19+/-4.15mg/g) and phenol (100.82+/-6.25mg/g) contents. SCEPP-5 also demonstrated the most potent inhibitory effect on H661 cell proliferation. Using DNA ladder and flow cytometry analysis, SCEPP-5 effectively induced H661 cell apoptosis as demonstrated by the accumulation of Sub-G1 peak and fragmentation of DNA. SCEPP-5 not only induced cell cycle arrest at S phase, it also up-regulated the expression of pro-apoptotic protein (Bax) and down-regulated the inhibitor of apoptosis protein (IAP). Furthermore, the apoptotic induction in H661 cells was found to associate with an elevated p53 protein expression, cytochrome c release, caspase-3 activation and PARP cleavage. Taken together, these results conclude that SCEPP-5 induced cell cycle arrest at S phase, and its apoptotic induction could be mediated through the p53-dependent pathway and modification of Bax and XIAP proteins expression. The results have also provided important pharmacological backgrounds for the potential use of PP supercritical fluid extract as products for cancer prevention.
Authors:
Shu-Jing Wu; Shun-Pang Chang; Doung-Liang Lin; Shyh-Shyan Wang; Fwu-Feuu Hou; Lean-Teik Ng
Related Documents :
21219449 - The joys and terrors of fast adaptation: new findings elucidate antibiotic resistance a...
15293879 - Complement activation by apoptotic cells occurs predominantly via igm and is limited to...
20576349 - All-trans retinoic acid downregulates alk in neuroblastoma cell lines and induces apopt...
19154459 - Bacille-calmette guèrin induces caspase-independent cell death in urothelial carcinoma...
7790399 - Apoptosis during hl-60 cell differentiation is closely related to a g0/g1 cell cycle ar...
14572459 - A role of p301l tau mutant in anti-apoptotic gene expression, cell cycle and apoptosis.
9601509 - Cell-to-cell movement of turnip crinkle virus is controlled by two small open reading f...
16253759 - Cooperative cell-growth inhibition by combination treatment with zd1839 (iressa) and tr...
18030389 - Cell-based high content screening using an integrated microfluidic device.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2009-2-4
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-8-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate Institute of Nutritional Health, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cytotoxicity and genotoxicity of superporous hydrogel containing interpenetrating polymer networks.
Next Document:  Gardenia jasminoides Ellis ethanol extract and its constituents reduce the risks of gastritis and re...