| Super-induction of Dicer-2 expression by alien double-stranded RNAs: an evolutionary ancient response to viral infection? | |
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MedLine Citation:
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PMID: 22562088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dicer-2 is a ribonuclease involved in the insect RNAi pathway. On attempting to knockdown Dicer-2 expression in the insect Blattella germanica by RNAi, we found that treatment with Dicer-2 dsRNA upregulated the targeted mRNA. This unexpected result was also observed after treating with a nucleopolyhedrovirus dsRNA. Experiments with this alien dsRNA showed an all-or-none response with a threshold for inducing Dicer-2 upregulation between 0.4 and 0.04 μg in terms of dsRNA concentration and between 50 and 20 bp in terms of dsRNA length. The response seems specific of dsRNA given that equivalent experiments carried out with dsDNA did not affect Dicer-2 expression. In insects, Dicer-2 is postulated to be a sensor of viral infections and a key antiviral defense element. The upregulation of Dicer-2 expression after dsRNA administration fits well with this sensor role, and the occurrence of this mechanism in B. germanica, a phylogenetically basal insect, suggests that sensing alien RNAs might be an ancestral function of Dicer-2 proteins. |
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Authors:
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Jesus Lozano; Eva Gomez-Orte; How-Jing Lee; Xavier Belles |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-06 |
Journal Detail:
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Title: Development genes and evolution Volume: 222 ISSN: 1432-041X ISO Abbreviation: Dev. Genes Evol. Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-05-31 Completed Date: 2012-08-30 Revised Date: 2012-10-18 |
Medline Journal Info:
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Nlm Unique ID: 9613264 Medline TA: Dev Genes Evol Country: Germany |
Other Details:
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Languages: eng Pagination: 229-35 Citation Subset: IM |
Affiliation:
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Institute of Evolutionary Biology, Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Evolution* Blattellidae / enzymology*, immunology*, metabolism, virology DNA, Viral / immunology* Phylogeny RNA, Double-Stranded / immunology* Ribonuclease III / metabolism* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Double-Stranded; EC 3.1.26.3/Ribonuclease III |
| Comments/Corrections | |
Erratum In:
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Dev Genes Evol. 2012 Sep;222(5):311 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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