| Sumoylation controls retinal progenitor proliferation by repressing cell cycle exit in Xenopus laevis. | |
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MedLine Citation:
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PMID: 20801111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Precisely controlled progenitor proliferation is essential for normal development. However, molecular mechanisms, which control the correct timing of cell cycle withdrawal during development, have been poorly understood. We show here that ubc9, a sumo-conjugating enzyme, controls the cell cycle exit of retinal progenitors. We found that ubc9 is highly expressed in retinal progenitors and stem cells in Xenopus embryos. Ubc9 physically and functionally associates with Xenopus hmgb3, which is required for retinal cell proliferation, and prolonged expression of ubc9 and hmgb3 results in suppression of the cell cycle exit of retinal progenitors in a sumoylation-dependent manner. Overexpression of ubc9 and hmgb3 decreased expression of the cell-cycle inhibitor p27(Xic1). Furthermore, progenitor proliferation is regulated, at least in part, by sumoylation of transcription factor Sp1. These results suggest a significant role of sumoylation for cell cycle regulation in retinal progenitors. |
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Authors:
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Koji Terada; Takahisa Furukawa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-27 |
Journal Detail:
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Title: Developmental biology Volume: 347 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-05 Completed Date: 2010-11-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 180-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Developmental Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Death Cell Lineage Cell Proliferation Central Nervous System / cytology, embryology, enzymology Consensus Sequence / genetics Cyclin-Dependent Kinase Inhibitor p27 / metabolism Embryo, Nonmammalian / cytology, enzymology Gene Expression Regulation, Developmental HMGB3 Protein / genetics, metabolism Lysine / metabolism Mice Neurons / cytology, metabolism Protein Binding RNA, Messenger / genetics, metabolism Retina / cytology*, embryology, metabolism* Small Ubiquitin-Related Modifier Proteins / metabolism* Sp1 Transcription Factor / metabolism Stem Cells / cytology*, metabolism Ubiquitin-Conjugating Enzymes / genetics, metabolism Xenopus laevis / embryology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/HMGB3 Protein; 0/RNA, Messenger; 0/Small Ubiquitin-Related Modifier Proteins; 0/Sp1 Transcription Factor; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 56-87-1/Lysine; EC 6.3.2.19/Ubiquitin-Conjugating Enzymes; EC 6.3.2.19/ubiquitin-conjugating enzyme UBC9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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