Document Detail

Sulphur and nitrogen nutrition influence the response of chickpea seeds to an added, transgenic sink for organic sulphur.
MedLine Citation:
PMID:  15234997     Owner:  NLM     Status:  MEDLINE    
In order to increase the concentration of the nutritionally essential sulphur amino acids in seed protein, a transgene encoding a methionine- and cysteine-rich protein, sunflower seed albumin (SSA), was transferred to chickpeas (Cicer arietinum L). Transgenic seeds that accumulated SSA contained more methionine and less oxidized sulphur than the controls, suggesting that additional demand for sulphur amino acids from the expression of the transgene stimulated sulphur assimilation. In addition, the activity of trypsin inhibitors, a known family of endogenous, sulphur-rich chickpea seed proteins, was diminished in transgenic, SSA-containing seeds compared with the non-transgenic controls. Together, these results indicate that the reduced sulphur sequestered into SSA was supplied partly by additional sulphur assimilation in the developing transgenic seeds, and partly by some diversion of sulphur amino acids from endogenous seed proteins. Growth of chickpeas on nutrient with a high sulphur-to-nitrogen ratio increased the total seed sulphur content and the accumulation of sulphur amino acids in the seeds, and partly mitigated the effect of SSA accumulation on the trypsin inhibitor amount. The results suggest that free methionine and O-acetylserine (OAS) acted as signals that modulated chickpea seed protein composition in response to the variation in sulphur demand, as well as in response to variation in the nitrogen and sulphur status of the plant.
Pasquale Chiaiese; Naoko Ohkama-Ohtsu; Lisa Molvig; Robert Godfree; Hugh Dove; Charles Hocart; Toru Fujiwara; T J V Higgins; Linda M Tabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-07-02
Journal Detail:
Title:  Journal of experimental botany     Volume:  55     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-09     Completed Date:  2005-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  1889-901     Citation Subset:  IM    
CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.
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MeSH Terms
Cicer / genetics*,  growth & development,  metabolism
Cysteine / metabolism
Methionine / metabolism
Nitrogen / metabolism*
Plant Proteins / genetics,  metabolism
Plants, Genetically Modified
Seeds / genetics*,  metabolism
Serine / analogs & derivatives*,  metabolism
Signal Transduction
Sulfur / metabolism*
Reg. No./Substance:
0/Plant Proteins; 4985-36-8/O-acetylserine; 52-90-4/Cysteine; 56-45-1/Serine; 63-68-3/Methionine; 7704-34-9/Sulfur; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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