Document Detail

Sulindac inhibited gene expression and activity of arylamine N-acetyltransferase and DNA-2-aminofluorene adduct formation in T24 human bladder tumor cells.
MedLine Citation:
PMID:  11828994     Owner:  NLM     Status:  MEDLINE    
We demonstrated in vivo that non-steroidal anti-inflammatory drugs including sulindac can act as inhibitors of urinary bladder carcinogenesis in rats. The aim of the present study was to determine whether sulindac affects arylamine N-acetyltransferase (NAT) activity and gene expression and DNA-2-aminofluorene adduct formation in the T24 human bladder tumor cell line. The NAT activity (N-acetylation of 2-aminofluorene) was measured by high performance liquid chromatography assaying for the amount of acetylated 2-aminofluorene and the remaining 2-aminofluorene (AF). The results demonstrated that NAT activity in T24 cells were inhibited by the sulindac in a dose-dependent manner. The apparent values of Km and Vmax of NAT from T24 cells were also decreased by sulindac. This inhibition was not competitive. The amount of DNA-AF adduct formation in T24 cells was also inhibited by sulindac. The data also demonstrated that sulindac inhibited the NAT mRNA level in T24 cells.
C C Yang; J G Chung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Urological research     Volume:  29     ISSN:  0300-5623     ISO Abbreviation:  Urol. Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-02-06     Completed Date:  2002-07-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364311     Medline TA:  Urol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  406-11     Citation Subset:  IM    
Department of Urology, China Medical College Hospital, Taichung, Taiwan, Republic of China.
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MeSH Terms
Aged, 80 and over
Antineoplastic Agents / pharmacology*
Arylamine N-Acetyltransferase / genetics*,  metabolism*
DNA Adducts / metabolism*
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Fluorenes / metabolism*
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Neoplastic / drug effects
RNA, Messenger / analysis
Sulindac / pharmacology*
Tumor Cells, Cultured / drug effects,  enzymology
Urinary Bladder Neoplasms*
Reg. No./Substance:
0/2-aminofluorene-DNA complex; 0/Antineoplastic Agents; 0/DNA Adducts; 0/Fluorenes; 0/RNA, Messenger; 38194-50-2/Sulindac; EC N-Acetyltransferase

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