Document Detail


Sulfur-nitrogen-carbon removal of Pseudomonas sp. C27 under sulfide stress.
MedLine Citation:
PMID:  23683698     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Pseudomonas sp. C27 is a facultative autotrophic bacterium that can effectively conduct mixotrophic and heterotrophic denitrification reactions using organic matters and sulfide as electron donors. There is no experimental confirmation on proteomic levels the pure C27 strain can have the capability to simultaneous removal of sulfide, nitrate and organic carbon from waters. The proteome in total C27 cell extracts was observed by two-dimensional gel electrophoresis. The 160mg/L sulfide up-regulated or specifically expressed succinate dehydrogenase, iron-sulfur protein, oxidoreductase, serine hydroxymethyltransferase, and iron superoxide dismutase for sulfide metabolism, 2-oxoglutarate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, NAD(+)-dependent aldehyde dehydrogenase, malate dehydrogenase and succinate dehydrogenase for carbon metabolism, and nitrous-oxide reductase and respiratory nitrate reductase for nitrogen metabolism. The study confirmed that the C27 strain has an effective enzyme system to conduct denitrifying sulfide removal reactions. Also, sulfide stress can enhance energy consumption rate and rates of nitrate reduction and sulfide oxidation by C27. Conversely, sulfide stress repressed the sulfate-reducing power of C27, evidenced by down-regulation or specific un-expression of sulfate ABC transporter, periplasmic sulfate-binding protein in the (C+N+S) sample.
Authors:
Hongliang Guo; Chuan Chen; Duu-Jong Lee; Aijie Wang; Nanqi Ren
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Publication Detail:
Type:  Journal Article     Date:  2013-04-12
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  53     ISSN:  1879-0909     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6-12     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
Affiliation:
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
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