Document Detail

Sulfur dioxide restores calcium homeostasis disturbance in rat with isoproterenol-induced myocardial injury.
MedLine Citation:
PMID:  22806909     Owner:  NLM     Status:  MEDLINE    
BACKGROUNDS: sulfur dioxide (SO₂) could relieve isoproterenol (ISO)-induced myocardial injury, while the mechanism is unclear. This study aims to explore whether the protective effect of SO₂ on ISO-induced myocardial injury was mediated by the restoration of calcium homeostasis disturbance in cardiomyocyte.
METHODS AND RESULTS: Rats were randomly divided into four groups: ISO group, ISO+SO₂ group, control group and SO₂ group. Content of Ca²⁺ in H9c2 cells was assayed using confocal microscope, and cardiac function parameters were measured by echocardiography. Plasma biochemical values and myocardial ultra-structure changes were measured. Meanwhile, the activity, protein and gene levels of sarcoplasmic reticulum Ca²⁺ ATPase (SERCA), and protein and phosphorylation of phospholamban (PLN) were detected. We found SO₂ derivatives could restore the decreased cardiac function, the abnormal lactate dehydrogenase, creatine kinase, alpha-hydroxybutyrate dehydrogenase, potassium, calcium, blood urea nitrogen and the damaged myocardial ultra-structure in rats, and regulate the increased Ca²⁺ content in H9c2 induced by ISO. In addition, compared with ISO group, the decreased activities, protein and mRNA level of SERCA, as well as the decreased protein phosphorylation level of PLN in myocardial tissues were increased in ISO+SO₂ group.
CONCLUSION: SO₂ derivatives might relieve calcium overload in association with the upregulating expression of SERCA and p-PLN/PLN by myocardial tissues in rats with ISO-induced myocardial injury.
Shanshan Chen; Junbao Du; Yinfang Liang; Rongyuan Zhang; Chaoshu Tang; Hongfang Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  27     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2012-11-13     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  1219-26     Citation Subset:  IM    
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MeSH Terms
Blotting, Western
Calcium / metabolism*
Cardiotonic Agents / toxicity
Disease Models, Animal
Heart Diseases / metabolism
Heart Injuries / chemically induced,  drug therapy*,  metabolism*
Homeostasis / physiology*
Isoproterenol / toxicity
Microscopy, Confocal
Microscopy, Electron, Transmission
Myocardium / metabolism
Rats, Wistar
Real-Time Polymerase Chain Reaction
Sulfur Dioxide / pharmacology*
Reg. No./Substance:
0/Cardiotonic Agents; 0UZA3422Q4/Sulfur Dioxide; L628TT009W/Isoproterenol; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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