Document Detail


Sulforaphane and its glutathione conjugate but not sulforaphane nitrile induce UDP-glucuronosyl transferase (UGT1A1) and glutathione transferase (GSTA1) in cultured cells.
MedLine Citation:
PMID:  12151360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucoraphanin in Brassica vegetables breaks down to either sulforaphane or sulforaphane nitrile depending on the conditions, and sulforaphane can be further conjugated with glutathione. Using a high-throughput microtitre plate assay and TaqMan real time quantitative RT-PCR to measure mRNA, we show that sulforaphane and its glutathione conjugate, but not the nitrile, increased significantly (P < 0.05) both UGT1A1 and GSTA1 mRNA levels in HepG2 and HT29 cells. These changes were accompanied by an increase in UGT1A1 protein, as assessed by immunoblotting, and a 2-8-fold increase in bilirubin glucuronidation. When treated together, the nitrile derivative did not affect sulforaphane induction. The induction of UGT1A1 and GSTA1 mRNA by sulforaphane was time and concentration dependent. The results show a functional induction of glucuronidation by sulforaphane but not sulforaphane nitrile, and show that the pathway of metabolism of glucosinolates in Brassica vegetables is important in determining the resulting biological and anticarcinogenic activities.
Authors:
Graham P Basten; Yongping Bao; Gary Williamson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  23     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-01     Completed Date:  2002-09-05     Revised Date:  2014-03-28    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  1399-404     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Bilirubin / metabolism
DNA Primers
Enzyme Induction
Glucuronides / metabolism
Glucuronosyltransferase / biosynthesis*,  genetics
Glutathione / chemistry*
Glutathione Transferase / biosynthesis*,  genetics
Humans
Isothiocyanates
Nitriles / chemistry
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Thiocyanates / chemistry,  pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Glucuronides; 0/Isothiocyanates; 0/Nitriles; 0/RNA, Messenger; 0/Thiocyanates; 4478-93-7/sulforafan; EC 2.4.1.-/UGT1A1 enzyme; EC 2.4.1.17/Glucuronosyltransferase; EC 2.5.1.18/Glutathione Transferase; GAN16C9B8O/Glutathione; RFM9X3LJ49/Bilirubin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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