| Sulforaphane and its glutathione conjugate but not sulforaphane nitrile induce UDP-glucuronosyl transferase (UGT1A1) and glutathione transferase (GSTA1) in cultured cells. | |
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MedLine Citation:
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PMID: 12151360 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucoraphanin in Brassica vegetables breaks down to either sulforaphane or sulforaphane nitrile depending on the conditions, and sulforaphane can be further conjugated with glutathione. Using a high-throughput microtitre plate assay and TaqMan real time quantitative RT-PCR to measure mRNA, we show that sulforaphane and its glutathione conjugate, but not the nitrile, increased significantly (P < 0.05) both UGT1A1 and GSTA1 mRNA levels in HepG2 and HT29 cells. These changes were accompanied by an increase in UGT1A1 protein, as assessed by immunoblotting, and a 2-8-fold increase in bilirubin glucuronidation. When treated together, the nitrile derivative did not affect sulforaphane induction. The induction of UGT1A1 and GSTA1 mRNA by sulforaphane was time and concentration dependent. The results show a functional induction of glucuronidation by sulforaphane but not sulforaphane nitrile, and show that the pathway of metabolism of glucosinolates in Brassica vegetables is important in determining the resulting biological and anticarcinogenic activities. |
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Authors:
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Graham P Basten; Yongping Bao; Gary Williamson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carcinogenesis Volume: 23 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-01 Completed Date: 2002-09-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
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Languages: eng Pagination: 1399-404 Citation Subset: IM |
Affiliation:
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Institute of Food Research, Norwich Research Park, Conley Lane, Norwich, NR4 7UA, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Bilirubin / metabolism DNA Primers Enzyme Induction Glucuronides / metabolism Glucuronosyltransferase / biosynthesis*, genetics Glutathione / chemistry* Glutathione Transferase / biosynthesis*, genetics Humans Nitriles / chemistry RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Thiocyanates / chemistry, pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Glucuronides; 0/Nitriles; 0/RNA, Messenger; 0/Thiocyanates; 4478-93-7/sulforafan; 635-65-4/Bilirubin; 70-18-8/Glutathione; EC 2.4.1.-/bilirubin uridine-diphosphoglucuronosyl transferase 1A1; EC 2.4.1.17/Glucuronosyltransferase; EC 2.5.1.18/Glutathione Transferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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